Vimentin mRNA Location Changes During Muscle Development

The mRNAs for some cytoskeletal proteins are localized, suggesting that mRNA for these proteins may concentrate at sites appropriate for assembly. To test this hypothesis, we observed vimentin mRNA in developing chicken muscle cultures by in situ hybridization with a digoxigenin-labeled DNA probe to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-04, Vol.90 (7), p.2724-2728
Main Authors: Cripe, L., Morris, E., Fulton, A. B.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal
Base Sequence
Biological and medical sciences
Cells
Cells, Cultured
Cellular biology
Chick Embryo
Codon
Costameres
DNA - genetics
Embryology: invertebrates and vertebrates. Teratology
Fibroblasts
Fundamental and applied biological sciences. Psychology
Laboratory animals
Messenger RNA
Molecular embryology
Molecular Sequence Data
Muscle fibers
Muscles - cytology
Muscles - embryology
Muscles - physiology
Muscular system
Myoblasts
Myofibrils
Oligodeoxyribonucleotides
Oligonucleotide Probes
Plasmids
Polymerase Chain Reaction - methods
Proteins
Ribonucleic acid
RNA
RNA, Messenger - analysis
RNA, Messenger - genetics
RNA, Messenger - metabolism
Somatic cells
Vimentin
Vimentin - analysis
Vimentin - biosynthesis
Vimentin - genetics
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Summary:The mRNAs for some cytoskeletal proteins are localized, suggesting that mRNA for these proteins may concentrate at sites appropriate for assembly. To test this hypothesis, we observed vimentin mRNA in developing chicken muscle cultures by in situ hybridization with a digoxigenin-labeled DNA probe to vimentin, detected by confocal microscopy using fluorescent anti-digoxigenin antibody. This method has submicrometer resolution. In developing muscle, vimentin mRNA was bipolar in young myoblasts, somewhat perinuclear in elongated myoblasts and spread fibroblasts, and diffuse in young and developing myotubes. In mature myotubes, vimentin mRNA occurred at costameres with vimentin protein. Localization of mRNA may prove important for assembling and maintaining differentiated cytoskeletal structures, as it is for organizing the embryo.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.7.2724