Quantitative characterization of cell synchronization in yeast

Metabolic oscillations in baker's yeast serve as a model system for synchronization of biochemical oscillations. Despite widespread interest, the complexity of the phenomenon has been an obstacle for a quantitative understanding of the cell synchronization process. In particular, when two yeast...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-07, Vol.104 (31), p.12732-12736
Main Authors: Danø, Sune, Madsen, Mads Find, Sørensen, Preben Graae
Format: Article
Language:English
Subjects:
acetaldehyde
Acetaldehyde - pharmacology
Biological Clocks - physiology
Biological Sciences
Biophysics
bioreactors
cell culture
cell synchronization
Cells
continuous-flow stirred-tank reactor
Cyanides
Facilitative glucose transport proteins
Fluorescence
glucose
Glucose - metabolism
Glycolysis
microbial growth
microbial physiology
Multiphase flow
nutrient availability
Oscillators
Phase shift
Resonance
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - drug effects
Synchronizers
Temperature
Yeast
Yeasts
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Summary:Metabolic oscillations in baker's yeast serve as a model system for synchronization of biochemical oscillations. Despite widespread interest, the complexity of the phenomenon has been an obstacle for a quantitative understanding of the cell synchronization process. In particular, when two yeast cell populations oscillating 180° out of phase are mixed, it appears as if the synchronization dynamics is too fast to be explained. We have probed the synchronization dynamics by forcing experiments in an open-flow reactor, and we find that acetaldehyde has a very strong synchronization effect that can account quantitatively for the classical mixing experiment. The fast synchronization dynamics is explained by a general synchronization mechanism, which is dominated by a fast amplitude response as opposed to the expected slow phase change. We also show that glucose can mediate this kind of synchronization, provided that the glucose transporter is not saturated. This makes the phenomenon potentially relevant for a broad range of cell types.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0702560104