Negative-Strand RNA Viruses: Genetic Engineering and Applications

The negative-strand RNA viruses are a broad group of animal viruses that comprise several important human pathogens, including influenza, measles, mumps, rabies, respiratory syncytial, Ebola, and hantaviruses. The development of new strategies to genetically manipulate the genomes of negative-strand...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-10, Vol.93 (21), p.11354-11358
Main Authors: Palese, Peter, Zheng, Hongyong, Engelhardt, Othmar G., Pleschka, Stephan, García-Sastre, Adolfo
Format: Article
Language:English
Subjects:
Animals
Cell lines
Chimera
Drug Design
Ebola virus
Gene therapy
Genetic Engineering
Genetic vectors
Genetically engineered microorganisms
Genome, Viral
hantavirus
Helper viruses
Humans
Influenza Vaccines
influenza virus
measles virus
mumps virus
Orthomyxoviridae
Orthomyxoviridae - genetics
Orthomyxoviridae - immunology
Papers from a Colloquium
Plasmids
Preventive medicine
rabies virus
respiratory syncytial virus
RNA
RNA viruses
RNA Viruses - genetics
RNA Viruses - pathogenicity
Transfection
Vaccines, Synthetic
Viral Vaccines
Virulence
Virus Diseases - immunology
Virus Diseases - prevention & control
Virus Diseases - virology
Viruses
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Summary:The negative-strand RNA viruses are a broad group of animal viruses that comprise several important human pathogens, including influenza, measles, mumps, rabies, respiratory syncytial, Ebola, and hantaviruses. The development of new strategies to genetically manipulate the genomes of negative-strand RNA viruses has provided us with new tools to study the structure--function relationships of the viral components and their contributions to the pathogenicity of these viruses. It is also now possible to envision rational approaches--based on genetic engineering techniques--to design live attenuated vaccines against some of these viral agents. In addition, the use of different negative-strand RNA viruses as vectors to efficiently express foreign polypeptides has also become feasible, and these novel vectors have potential applications in disease prevention as well as in gene therapy.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.21.11354