Optimization of explants sterilizations intended for In vitro culture of sp. Prunus avium

A balance between concentration and time must be determined empirically for each type of explants because of phytotoxicity.[...]the aim of this study was to assess the effective prunus explant- surface sterilization. 2.Material and method The study was conducted at the Micro propagation Laboratory,...

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Bibliographic Details
Published in:Albanian journal of agricultural sciences 2017-01, p.571-574
Main Authors: Gashi, Avdirrahman, Thomaj, Tokli, Mitrushi, Taulant, Kukali, Edlira
Format: Article
Language:English
Subjects:
Agricultural practices
Bacteria
Bleaches
Data acquisition
Decantation
Distilled water
Drinking water
Ethanol
Explants
Laboratories
Mercury compounds
Microorganisms
Optimization
Prunus avium
Prunus mahaleb
Reagents
Sodium
Sodium hypochlorite
Statistical analysis
Sterilization
Trees
Variance analysis
Water pollution
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Summary:A balance between concentration and time must be determined empirically for each type of explants because of phytotoxicity.[...]the aim of this study was to assess the effective prunus explant- surface sterilization. 2.Material and method The study was conducted at the Micro propagation Laboratory, of Agricultural University of Tirana from October 2015 to January 2016.[3] After decanting sterilizing solutions under safe conditions, the explants were washed three times each for 5 min with sterile distilled water to remove traces of densifectantes. 2.2 Data collection and Statistical analysis The sterilization experiments data recorded include the number of contaminated, dead and survived.[6] Explants must be surface sterilized only by treatment with disinfectant solution at suitable concentrations for a specified period [7].[...]0.25%NaOC llocal bleach for 15 min exposure time was found to be the most effective one for explants taken from shoots. 4.Conclusions *Buds isolation after sterilization 0.01% HgCl2 for 15 min are characterized by higher survival rate, respectively 95% for P. mahaleb and 92% for P. avium (rootstocks) *Sodium Hypochlorite Concentration of 0.15 %, 15 min was does result effective in sterilization of explants, isolated from trees on the field. *I must point out that, the explants are isolated from the trees of the field with high levels of pollution and consequently they needed to sterilization more powerful with reagents such as mercury chloride. 5.
ISSN:2218-2020