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Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14

The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete mole...

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Published in:	Proceedings of the National Academy of Sciences - PNAS 2010-03, Vol.107 (11), p.4919-4924
Main Authors:	Koopman, Frank, Wierckx, Nick, de Winde, Johannes H., Ruijssenaars, Harald J., Ingram, Lonnie O'Neal
Format:	Article
Language:	English
Subjects:	Alcohols Aldehydes Bacteria Biochemical pathways Biochemistry Biological Sciences Carbon Cell extracts Cupriavidus - cytology Cupriavidus - genetics Cupriavidus - growth & development Cupriavidus - metabolism Dehydrogenases DNA Transposable Elements - genetics Enzymes Furaldehyde - analogs & derivatives Furaldehyde - metabolism Genes Genes, Bacterial - genetics Genetics Metabolic Networks and Pathways Metabolism Molecules Mutagenesis - genetics Oxidoreductases - metabolism Phenotype Pseudomonas putida Pseudomonas putida - metabolism Studies Transposons
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creator	Koopman, Frank Wierckx, Nick de Winde, Johannes H. Ruijssenaars, Harald J. Ingram, Lonnie O'Neal
description	The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl)furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of the pathway by which Cupriavidus basilensis HMF14 metabolizes HMF and furfural. The identification of this pathway enabled the construction of an HMF and furfural-metabolizing Pseudomonas putida. The genetic information obtained furthermore enabled us to predict the HMF and furfural degrading capabilities of sequenced bacterial species that had not previously been connected to furanic aldehyde metabolism. These results pave the way for in situ detoxification of lignocellulosic hydrolysates, which is a major step toward improved efficiency of utilization of lignocellulosic feedstock.
doi_str_mv	10.1073/pnas.0913039107
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subjects	Alcohols Aldehydes Bacteria Biochemical pathways Biochemistry Biological Sciences Carbon Cell extracts Cupriavidus - cytology Cupriavidus - genetics Cupriavidus - growth & development Cupriavidus - metabolism Dehydrogenases DNA Transposable Elements - genetics Enzymes Furaldehyde - analogs & derivatives Furaldehyde - metabolism Genes Genes, Bacterial - genetics Genetics Metabolic Networks and Pathways Metabolism Molecules Mutagenesis - genetics Oxidoreductases - metabolism Phenotype Pseudomonas putida Pseudomonas putida - metabolism Studies Transposons
title	Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14
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