Continuous Network of Endoplasmic Reticulum in Cerebellar Purkinje Neurons

Purkinje neurons in rat cerebellar slices injected with an oil drop saturated with 1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate [DiIC16(3) or DiI] to label the endoplasmic reticulum were observed by confocal microscopy. DiI spread throughout the cell body and den...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1994-08, Vol.91 (16), p.7510-7514
Main Authors: Terasaki, M, Slater, N T, Fein, A, Schmidek, A, Reese, T S
Format: Article
Language:English
Subjects:
Animals
Axons
Carbocyanines
Cell membranes
Cells, Cultured
Cerebellum - cytology
Dendrites
Diffusion
Dyes
Electron microscopy
Endoplasmic reticulum
Endoplasmic Reticulum - ultrastructure
Fluorescent Dyes
Hippocampus - cytology
Histocytochemistry
In Vitro Techniques
Intracellular membranes
Microscopes
Neurology
Neurons
Purkinje cells
Purkinje Cells - ultrastructure
Rats
Rats, Sprague-Dawley
Rodents
Staining and Labeling
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Summary:Purkinje neurons in rat cerebellar slices injected with an oil drop saturated with 1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate [DiIC16(3) or DiI] to label the endoplasmic reticulum were observed by confocal microscopy. DiI spread throughout the cell body and dendrites and into the axon. DiI spreading is due to diffusion in a continuous bilayer and is not due to membrane trafficking because it also spreads in fixed neurons. DiI stained such features of the endoplasmic reticulum as densities at branch points, reticular networks in the cell body and dendrites, nuclear envelope, spines, and aggregates formed during anoxia in low extracellular Ca2+. In cultured rat hippocampal neurons, where optical conditions provide more detail, DiI labeled a clearly delineated network of endoplasmic reticulum in the cell body. We conclude that there is a continuous compartment of endoplasmic reticulum extending from the cell body throughout the dendrites. This compartment may coordinate and integrate neuronal functions.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.16.7510