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Rho Protein Regulates Tight Junctions and Perijunctional Actin Organization in Polarized Epithelia
The rho family of GTP-binding proteins regulates actin filament organization. In unpolarized mammalian cells, rho proteins regulate the assembly of actin-containing stress fibers at the cell-matrix interface. Polarized epithelial cells, in contrast, are tall and cylindrical with well developed inter...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1995-11, Vol.92 (23), p.10629-10633 |
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| Main Authors: | , , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c591t-1c57ea44692367c78525647d4e49b20041054f3fc18009896c4ed693de22b3463 |
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| container_end_page | 10633 |
| container_issue | 23 |
| container_start_page | 10629 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 92 |
| creator | Nusrat, A. Giry, M. Turner, J. R. Colgan, S. P. Parkos, C. A. Carnes, D. Lemichez, E. Boquet, P. Madara, J. L. |
| description | The rho family of GTP-binding proteins regulates actin filament organization. In unpolarized mammalian cells, rho proteins regulate the assembly of actin-containing stress fibers at the cell-matrix interface. Polarized epithelial cells, in contrast, are tall and cylindrical with well developed intercellular tight junctions that permit them to behave as biologic barriers. We report that rho regulates filamentous actin organization preferentially in the apical pole of polarized intestinal epithelial cells and, in so doing, influences the organization and permeability of the associated apical tight junctions. Thus, barrier function, which is an essential characteristic of columnar epithelia, is regulated by rho. |
| doi_str_mv | 10.1073/pnas.92.23.10629 |
| format | article |
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R.</au><au>Colgan, S. P.</au><au>Parkos, C. A.</au><au>Carnes, D.</au><au>Lemichez, E.</au><au>Boquet, P.</au><au>Madara, J. L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rho Protein Regulates Tight Junctions and Perijunctional Actin Organization in Polarized Epithelia</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1995-11-07</date><risdate>1995</risdate><volume>92</volume><issue>23</issue><spage>10629</spage><epage>10633</epage><pages>10629-10633</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The rho family of GTP-binding proteins regulates actin filament organization. In unpolarized mammalian cells, rho proteins regulate the assembly of actin-containing stress fibers at the cell-matrix interface. Polarized epithelial cells, in contrast, are tall and cylindrical with well developed intercellular tight junctions that permit them to behave as biologic barriers. We report that rho regulates filamentous actin organization preferentially in the apical pole of polarized intestinal epithelial cells and, in so doing, influences the organization and permeability of the associated apical tight junctions. Thus, barrier function, which is an essential characteristic of columnar epithelia, is regulated by rho.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>7479854</pmid><doi>10.1073/pnas.92.23.10629</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1995-11, Vol.92 (23), p.10629-10633 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_journals_201332450 |
| source | PubMed (Medline); JSTOR Archival Journals and Primary Sources Collection【Remote access available】 |
| subjects | Actins Actins - physiology Actins - ultrastructure ADP Ribose Transferases - metabolism Biochemistry Blotting, Western Botulinum Toxins Cadherins Cadherins - metabolism Cell Adhesion Cell Communication Cell lines Cell Polarity - physiology Cells Cells, Cultured Cellular biology Epithelial Cells Fluorescent Antibody Technique GTP-Binding Proteins - genetics GTP-Binding Proteins - metabolism Humans Intestines - cytology Membrane Proteins - metabolism Membranes Microfilaments Phosphoproteins - metabolism Physiological regulation Precipitin Tests Proteins Recombinant Proteins - metabolism rho GTP-Binding Proteins rhoC GTP-Binding Protein Signal Transduction Stress fibers Tight junctions Tight Junctions - physiology Tight Junctions - ultrastructure Toxins Zonula Occludens-1 Protein |
| title | Rho Protein Regulates Tight Junctions and Perijunctional Actin Organization in Polarized Epithelia |
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