Functional role of lncRNA LOC101927497 in N-methyl-N′-nitro-N-nitrosoguanidine-induced malignantly transformed human gastric epithelial cells

Evidence shows that aberrant expression of long non-coding RNA (lncRNA) is closely associated with tumor development and progression. However, the role of lncRNA in environmental carcinogen induced gastric tumorigenesis remains largely unknown. This study aimed at investigating the function role of...

Full description

Saved in:
Bibliographic Details
Published in:Life sciences (1973) 2018-01, Vol.193, p.93-103
Main Authors: Luo, Yuanwei, Liang, Min, Yao, Wenxia, Liu, Jifang, Niu, Qiuling, Chen, Jitao, Liu, Zhaoyu, Li, Ming, Shi, Boyun, Pan, Jinhui, Zhou, Lin, Zhou, Xinke
Format: Article
Language:English
Subjects:
Antisense RNA
Carcinogens
Cell proliferation
Cells
Chemical carcinogenesis
Epithelial cells
Gastric cancer
Gene expression
LOC101927497
Long non-coding RNA
Lymphocytes
Lymphocytes T
MNNG
N-Methyl-N'-nitro-N-nitrosoguanidine
Next-generation sequencing
Non-coding RNA
Phenotypes
Purification
Ribonucleic acid
RNA
Tumor suppressor genes
Tumorigenesis
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Evidence shows that aberrant expression of long non-coding RNA (lncRNA) is closely associated with tumor development and progression. However, the role of lncRNA in environmental carcinogen induced gastric tumorigenesis remains largely unknown. This study aimed at investigating the function role of lncRNA in N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) induce malignantly transformed human gastric epithelial cells. In this study, high-throughput sequencing and qRT-PCR assay revealed marked downregulation of lncRNA LOC101927497 in the malignant transformed gastric epithelial cells induced by MNNG (GES-1-T cells), gain-of-function and loss-of-function assays showed that LOC101927497 can suppress the proliferation and migration of GES-1-T cells in vitro. RNA antisense purification experiment showed that LOC101927497 interacted with miR-574-5p in GES-1-T cells the most obvious. Further studies suggested that LOC101927497 may function as a tumor suppressor by interacting with miR-574-5p. LncRNA LOC101927497 functions as a suppressor by interacting with miR-574-5p, thus inhibiting the malignant phenotype of GES-1-T cells. To the best of our knowledge, this is the first study to demonstrate the role of lncRNA in MNNG-induced gastric tumorigenesis, and it will provide new insights into the role of lncRNA in environmental carcinogen-induced gastric cancer.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2017.12.007