Crystal Structure of a Human TATA Box-Binding Protein/TATA Element Complex

The TATA box-binding protein (TBP) is required by all three eukaryotic RNA polymerases for correct initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs. The cocrystal structure of the C-terminal/core region of human TBP complexed with the TATA element of the adenovir...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-05, Vol.93 (10), p.4862-4867
Main Authors: Nikolov, Dimitar B., Chen, Hua, Halay, Elaine D., Hoffmann, Alexander, Roeder, Robert G., Burley, Stephen K.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Arabidopsis - chemistry
Arabidopsis thaliana
Atomic interactions
Atoms
Base Sequence
Conserved Sequence
Crystallography
Crystallography, X-Ray
DNA
DNA - chemistry
DNA - genetics
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Humans
Hydrogen bonds
Models, Molecular
Molecular biology
Molecular Sequence Data
Molecular Structure
Molecules
Nucleic Acid Conformation
Phosphates
Plant Proteins - chemistry
Protein Conformation
Proteins
TATA Box
TATA-Box Binding Protein
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
Yeasts
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The TATA box-binding protein (TBP) is required by all three eukaryotic RNA polymerases for correct initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs. The cocrystal structure of the C-terminal/core region of human TBP complexed with the TATA element of the adenovirus major late promoter has been determined at 1.9 angstrom resolution. Structural and functional analyses of the protein-DNA complex are presented, with a detailed comparison to our 1.9- angstrom -resolution structure of Arabidopsis thaliana TBP2 bound to the same TATA box.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.10.4862