Regulation of the murine renal vitamin D receptor by 1,25-dihydroxyvitamin D3 and calcium

• Published in: Proceedings of the National Academy of Sciences - PNAS 2003-08, Vol.100 (17), p.9733-9737
• Main Authors: Healy, Kevin D, Zella, Julia B, Prahl, Jean M, DeLuca, Hector F
• Format: Article
• Language: English
• Subjects: 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - genetics; 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - metabolism; Animals; Base Sequence; Biological Sciences; Calcitriol - administration & dosage; Calcitriol - pharmacology; Calcium; Calcium, Dietary - administration & dosage; Calcium, Dietary - pharmacology; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; DNA - genetics; Female; Gene Expression Regulation - drug effects; Kidney - drug effects; Kidney - metabolism; Kidneys; Male; Mice; Mice, Inbred NOD; Pregnancy; Receptors, Calcitriol - genetics; Receptors, Calcitriol - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Steroid Hydroxylases - genetics; Steroid Hydroxylases - metabolism; Vitamin D; Vitamin D3 24-Hydroxylase
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1633774100

Renal vitamin D receptor (VDR) is required for 1,25-dihydroxyvitamin D 3 -[1,25(OH) 2 D 3 ]-induced renal reabsorption of calcium and for 1,25(OH) 2 D 3 -induced 1,25(OH) 2 D 3 24-hydroxylase. The long-term effect of vitamin D and dietary calcium on the expression of renal VDR was examined in the nonobese diabetic mouse. Vitamin D-deficient and vitamin D-replete mice were maintained on diets containing 0.02%, 0.25%, 0.47%, and 1.20% calcium with or without 50 ng of 1,25(OH) 2 D 3 per day. Vitamin D-replete mice on a 1.20% calcium diet had renal VDR levels of 165 fmol/mg protein. Calcium restriction caused renal VDR levels to decrease to