In vivo Telomere Dynamics of Human Hematopoietic Stem Cells

Aging in vivo and cell division in vitro are associated with telomere shortening. Several lines of evidence suggest that telomere length may be a good predictor of the long term replicative capacity of cells. To investigate the natural fate of chromosome telomeres of hematopoietic stem cells in vivo...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-12, Vol.94 (25), p.13782-13785
Main Authors: Notaro, Rosario, Cimmino, Amelia, Tabarini, Diane, Rotoli, Bruno, Luzzatto, Lucio
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological Sciences
Blood
Blood cells
Bone marrow
Bone marrow cells
Bone Marrow Transplantation - pathology
Cell Division
Cell lines
Cellular Senescence
Child
Chromosomes
Chronic myeloid leukemia
Deoxyribonucleic acid
DNA
DNA probes
Female
Genetics
Hematopoiesis
Hematopoietic stem cells
Hematopoietic Stem Cells - ultrastructure
Humans
Male
Stem cells
Telomere - ultrastructure
Telomeres
Time Factors
Tissue Donors
Transplants & implants
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Summary:Aging in vivo and cell division in vitro are associated with telomere shortening. Several lines of evidence suggest that telomere length may be a good predictor of the long term replicative capacity of cells. To investigate the natural fate of chromosome telomeres of hematopoietic stem cells in vivo, we measured the telomere length of peripheral blood granulocytes from 11 fully engrafted bone marrow transplant recipients and from their respective donors. In 10 of 11 donor-recipient pairs, the telomere length was significantly reduced in the recipient and the extent of reduction correlated inversely with the number of nucleated cells infused. These data provide internally controlled in vivo evidence that, concomitantly with their proliferation, hematopoietic stem cells lose telomere length; it is possible that, as a result, their proliferative potential is reduced. These findings must be taken into account when developing new protocols in which few stem cells are used for bone marrow transplantation or for gene therapy.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.25.13782