The Relationships between Notochord and Floor Plate in Vertebrate Development Revisited

By using the quail-chicken chimera system, we have previously shown that during development of the spinal cord, floor plate cells are inserted between neural progenitors giving rise to the alar plates. These cells are derived from the regressing Hensen's node or cordoneural hinge (HN-CNH). This...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-09, Vol.95 (20), p.11733-11738
Main Authors: M.-A. Teillet, Lapointe, F., Le Douarin, N. M.
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Biology
Cell separation
Chick Embryo
Chickens
Chimera
Chimeras
Developmental biology
DNA-Binding Proteins - genetics
Embryonic Induction - genetics
Embryonic structures
Embryos
Endoderm
Endoderm - cytology
Endoderm - metabolism
Gene Expression Regulation, Developmental
Hedgehog Proteins
Hepatocyte Nuclear Factor 3-beta
In Situ Hybridization
Neurons
Notochord - cytology
Notochord - embryology
Notochord - metabolism
Nuclear Proteins - genetics
Proteins - genetics
Quail
Quails
Spinal cord
Spinal Cord - cytology
Spinal Cord - embryology
Spinal Cord - metabolism
Tissue grafting
Trans-Activators
Transcription Factors
Vertebrates
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Summary:By using the quail-chicken chimera system, we have previously shown that during development of the spinal cord, floor plate cells are inserted between neural progenitors giving rise to the alar plates. These cells are derived from the regressing Hensen's node or cordoneural hinge (HN-CNH). This common population of HN-CNH cells gives rise to three types of midline descendants: notochord, floor plate, and dorsal endoderm. Here we find that HNF3β , an important gene in the development of the midline structures, is continuously expressed in the HN-CNH cells and their derivatives, floor plate, notochord, and dorsal endoderm. Experiments in which the notochord was removed in the posterior region of either normal chicken or of quail-chicken chimeras in which a quail HN had been grafted showed that the floor plate develops in a cell-autonomous manner in the absence of notochord. Absence of floor plate observed at the posterior level of the excision results from removal of HN-CNH material, including the future floor plate, and not from the lack of an inductive signal of notochord origin.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.20.11733