In vivo Transposition of Minos, A Drosophila Mobile Element, in Mammalian Tissues

Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Droso...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-09, Vol.98 (20), p.11474-11478
Main Authors: Zagoraiou, Laskaro, Drabek, Dubravka, Alexaki, Sofia, Guy, Jacky A., Klinakis, Apostolos G., Langeveld, An, Skavdis, George, Mamalaki, Clio, Grosveld, Frank, Savakis, Charalambos
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological Sciences
CD2 antigen
Cell Line
Chromosome Mapping
Chromosomes
DNA
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila hydei
Experiments
Female
Genetic transposition
Genetics
Male
Mammals
Mice
Mice, Transgenic
Mutagenesis, Insertional
Plasmids
Polymerase chain reaction
Spleen
Spleen - enzymology
T lymphocytes
Tc1/Mariner transposon family
Telomere - genetics
Thymus Gland - enzymology
Tissues
Transfection
Transgenic animals
transposase
Transposases - genetics
Transposases - metabolism
Transposon mutagenesis
Transposons
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Summary:Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Drosophila hydei, to transpose in mouse tissues. Two transgenic mouse lines were crossed, one expressing Minos transposase in lymphocytes under the control of the CD2 promoter/locus control region and another carrying a nonautonomous Minos transposon. Only mice containing both transgenes show excision of the transposon and transposition into new chromosomal sites in thymus and spleen cells. In addition, expression of Minos transposase in embryonic fibroblast cell lines derived from a transposon-carrying transgenic mouse resulted in excision of the transposon. These results are a first step toward a reversible insertional mutagenesis system in the mouse, opening the way to develop powerful technologies for functional genomic analysis in mammals.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.201392398