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In vivo Transposition of Minos, A Drosophila Mobile Element, in Mammalian Tissues
Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Droso...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 2001-09, Vol.98 (20), p.11474-11478 |
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| Main Authors: | , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c520t-24ef619798d6f13d75f2e3bd4255ad3510c765639bcf925c17711690c638a4b73 |
| container_end_page | 11478 |
| container_issue | 20 |
| container_start_page | 11474 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 98 |
| creator | Zagoraiou, Laskaro Drabek, Dubravka Alexaki, Sofia Guy, Jacky A. Klinakis, Apostolos G. Langeveld, An Skavdis, George Mamalaki, Clio Grosveld, Frank Savakis, Charalambos |
| description | Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Drosophila hydei, to transpose in mouse tissues. Two transgenic mouse lines were crossed, one expressing Minos transposase in lymphocytes under the control of the CD2 promoter/locus control region and another carrying a nonautonomous Minos transposon. Only mice containing both transgenes show excision of the transposon and transposition into new chromosomal sites in thymus and spleen cells. In addition, expression of Minos transposase in embryonic fibroblast cell lines derived from a transposon-carrying transgenic mouse resulted in excision of the transposon. These results are a first step toward a reversible insertional mutagenesis system in the mouse, opening the way to develop powerful technologies for functional genomic analysis in mammals. |
| doi_str_mv | 10.1073/pnas.201392398 |
| format | article |
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Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Drosophila hydei, to transpose in mouse tissues. Two transgenic mouse lines were crossed, one expressing Minos transposase in lymphocytes under the control of the CD2 promoter/locus control region and another carrying a nonautonomous Minos transposon. Only mice containing both transgenes show excision of the transposon and transposition into new chromosomal sites in thymus and spleen cells. In addition, expression of Minos transposase in embryonic fibroblast cell lines derived from a transposon-carrying transgenic mouse resulted in excision of the transposon. These results are a first step toward a reversible insertional mutagenesis system in the mouse, opening the way to develop powerful technologies for functional genomic analysis in mammals.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.201392398</identifier><identifier>PMID: 11562481</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Base Sequence ; Biological Sciences ; CD2 antigen ; Cell Line ; Chromosome Mapping ; Chromosomes ; DNA ; Drosophila ; Drosophila - embryology ; Drosophila - genetics ; Drosophila hydei ; Experiments ; Female ; Genetic transposition ; Genetics ; Male ; Mammals ; Mice ; Mice, Transgenic ; Mutagenesis, Insertional ; Plasmids ; Polymerase chain reaction ; Spleen ; Spleen - enzymology ; T lymphocytes ; Tc1/Mariner transposon family ; Telomere - genetics ; Thymus Gland - enzymology ; Tissues ; Transfection ; Transgenic animals ; transposase ; Transposases - genetics ; Transposases - metabolism ; Transposon mutagenesis ; Transposons</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2001-09, Vol.98 (20), p.11474-11478</ispartof><rights>Copyright 1993-2001 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 25, 2001</rights><rights>Copyright © 2001, The National Academy of Sciences 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-24ef619798d6f13d75f2e3bd4255ad3510c765639bcf925c17711690c638a4b73</citedby><cites>FETCH-LOGICAL-c520t-24ef619798d6f13d75f2e3bd4255ad3510c765639bcf925c17711690c638a4b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/98/20.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3056739$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3056739$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11562481$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zagoraiou, Laskaro</creatorcontrib><creatorcontrib>Drabek, Dubravka</creatorcontrib><creatorcontrib>Alexaki, Sofia</creatorcontrib><creatorcontrib>Guy, Jacky A.</creatorcontrib><creatorcontrib>Klinakis, Apostolos G.</creatorcontrib><creatorcontrib>Langeveld, An</creatorcontrib><creatorcontrib>Skavdis, George</creatorcontrib><creatorcontrib>Mamalaki, Clio</creatorcontrib><creatorcontrib>Grosveld, Frank</creatorcontrib><creatorcontrib>Savakis, Charalambos</creatorcontrib><title>In vivo Transposition of Minos, A Drosophila Mobile Element, in Mammalian Tissues</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in Drosophila. Transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. We tested the ability of Minos, a DNA transposon from Drosophila hydei, to transpose in mouse tissues. Two transgenic mouse lines were crossed, one expressing Minos transposase in lymphocytes under the control of the CD2 promoter/locus control region and another carrying a nonautonomous Minos transposon. Only mice containing both transgenes show excision of the transposon and transposition into new chromosomal sites in thymus and spleen cells. In addition, expression of Minos transposase in embryonic fibroblast cell lines derived from a transposon-carrying transgenic mouse resulted in excision of the transposon. These results are a first step toward a reversible insertional mutagenesis system in the mouse, opening the way to develop powerful technologies for functional genomic analysis in mammals.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>CD2 antigen</subject><subject>Cell Line</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>DNA</subject><subject>Drosophila</subject><subject>Drosophila - embryology</subject><subject>Drosophila - genetics</subject><subject>Drosophila hydei</subject><subject>Experiments</subject><subject>Female</subject><subject>Genetic transposition</subject><subject>Genetics</subject><subject>Male</subject><subject>Mammals</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Mutagenesis, Insertional</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Spleen</subject><subject>Spleen - enzymology</subject><subject>T lymphocytes</subject><subject>Tc1/Mariner transposon family</subject><subject>Telomere - genetics</subject><subject>Thymus Gland - enzymology</subject><subject>Tissues</subject><subject>Transfection</subject><subject>Transgenic animals</subject><subject>transposase</subject><subject>Transposases - genetics</subject><subject>Transposases - metabolism</subject><subject>Transposon mutagenesis</subject><subject>Transposons</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAUhS0EokNhywqBxaJi0Qx-PyQ2VVugUkcIaVhbTsahHjl2Gicj-Pc4mmF4LGDlxf3Oucf3APAcoyVGkr7to81LgjDVhGr1ACww0rgSTKOHYIEQkZVihJ2AJzlvEUKaK_QYnGDMBWEKL8Dnmwh3fpfgerAx9yn70acIUwtXPqZ8Di_g1ZBy6u98sHCVah8cvA6uc3E8hz7Cle06G7yNcO1znlx-Ch61NmT37PCegi_vr9eXH6vbTx9uLi9uq4YTNFaEuVZgLbXaiBbTjeQtcbTeMMK53VCOUSMFF1TXTasJb7CUGAuNGkGVZbWkp-Dd3ref6s5tmhJosMH0g-_s8N0k682fk-jvzNe0M1xJzor87CAf0n2JPZrO58aFYKNLUzZlmy57-X9BrLAQiqICvv4L3KZpiOUGZi6IS65nt-UeaspV8-DaY2CMzNyomRs1x0aL4OXv3_yFHyoswJsDMAt_jrUqHgVikpl2CmF038aCvvo3WogXe2KbxzQcEYq4kFTTH0sovPU</recordid><startdate>20010925</startdate><enddate>20010925</enddate><creator>Zagoraiou, Laskaro</creator><creator>Drabek, Dubravka</creator><creator>Alexaki, Sofia</creator><creator>Guy, Jacky A.</creator><creator>Klinakis, Apostolos G.</creator><creator>Langeveld, An</creator><creator>Skavdis, George</creator><creator>Mamalaki, Clio</creator><creator>Grosveld, Frank</creator><creator>Savakis, Charalambos</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010925</creationdate><title>In vivo Transposition of Minos, A Drosophila Mobile Element, in Mammalian Tissues</title><author>Zagoraiou, Laskaro ; 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2001-09, Vol.98 (20), p.11474-11478 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_journals_201357595 |
| source | JSTOR Archival Journals and Primary Sources Collection; PubMed Central |
| subjects | Animals Base Sequence Biological Sciences CD2 antigen Cell Line Chromosome Mapping Chromosomes DNA Drosophila Drosophila - embryology Drosophila - genetics Drosophila hydei Experiments Female Genetic transposition Genetics Male Mammals Mice Mice, Transgenic Mutagenesis, Insertional Plasmids Polymerase chain reaction Spleen Spleen - enzymology T lymphocytes Tc1/Mariner transposon family Telomere - genetics Thymus Gland - enzymology Tissues Transfection Transgenic animals transposase Transposases - genetics Transposases - metabolism Transposon mutagenesis Transposons |
| title | In vivo Transposition of Minos, A Drosophila Mobile Element, in Mammalian Tissues |
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