A cGMP-Signaling Pathway in a Subset of Olfactory Sensory Neurons

It is well established that signal transduction in sensory neurons of the rat olfactory epithelium involves a cAMP-signaling pathway. However, a small number of olfactory neurons specifically express cGMP-signaling components, namely a guanylyl cyclase (GC-D) and a cGMP-stimulated phosphodiesterase...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-09, Vol.97 (19), p.10595-10600
Main Authors: Meyer, Mike R., Angele, Albert, Kremmer, Elisabeth, Kaupp, U. Benjamin, Muller, Frank
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies
Biological Sciences
Biology
Cell Line
Cells
Cilia
Cloning, Molecular
Complementary DNA
Cyclic GMP - metabolism
Cyclic Nucleotide-Gated Cation Channels
DNA, Complementary
Fluorescent antibody techniques
Humans
Immunohistochemistry
Ion Channels - genetics
Ligands
Molecular Sequence Data
Neurology
Neurons
Nose
Olfactory Receptor Neurons - metabolism
Phosphoric Diester Hydrolases - metabolism
Polymerase chain reaction
Proteins
Rats
Rats, Sprague-Dawley
Receptors
Senses
Signal Transduction
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Summary:It is well established that signal transduction in sensory neurons of the rat olfactory epithelium involves a cAMP-signaling pathway. However, a small number of olfactory neurons specifically express cGMP-signaling components, namely a guanylyl cyclase (GC-D) and a cGMP-stimulated phosphodiesterase (PDE2). Here, we show that this subset of olfactory neurons expressing GC-D and PDE2 does also express the subunit of a cGMP-selective cyclic nucleotidegated (CNG) channel that has been previously identified in cone photoreceptors. Further, components of the prototypical cAMP-signaling pathway could not be detected in this subpopulation of cells. These results imply that these neurons use an alternative signaling pathway, with cGMP as the intracellular messenger, and that, in these cells, the receptor current is initiated by the opening of cGMP-gated channels.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.97.19.10595