The Role of Zinc in the Binding of Killer Cell Ig-like Receptors to Class I MHC Proteins

The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During experiments to investigate the biochemistry of receptor-ligand binding in more detail, the kinetic parameter...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-02, Vol.98 (4), p.1734-1739
Main Authors: Valés-Gómez, Mar, Erskine, Robert A., Deacon, Matthew P., Strominger, Jack L., Reyburn, Hugh T.
Format: Article
Language:English
Subjects:
Biological Sciences
Cells
Dimers
Divalent cations
histocompatibility antigen HLA
Histocompatibility Antigens Class I - immunology
HLA C antigens
HLA-C Antigens - immunology
Humans
Immunology
killer cell immunoglobulin-like receptors
Killer Cells, Natural - immunology
Kinetics
Ligands
Magnesium - immunology
Molecular interactions
Molecules
Natural killer cells
Proteins
Receptors
Receptors, Immunologic - immunology
Receptors, KIR
Zinc
Zinc - immunology
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Summary:The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During experiments to investigate the biochemistry of receptor-ligand binding in more detail, the kinetic parameters of the interaction were observed to alter dramatically in the presence of Zn2+but not other divalent cations. The basis of this phenomenon is Zn2+-induced multimerization of the KIR molecules as demonstrated by BIAcore, analytical ultracentrifugation, and chemical cross-linking experiments. Zn2+-dependent multimerization of KIR may be critical for formation of the clusters of KIR and HLA-C molecules, the "natural killer (NK) cell immune synapse," observed at the site of contact between the NK cell and target cell.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.041618298