Broad-Spectrum Biofilm Inhibition by a Secreted Bacterial Polysaccharide

The development of surface-attached biofilm bacterial communities is considered an important source of nosocomial infections. Recently, bacterial interference via signaling molecules and surface active compounds was shown to antagonize biofilm formation, suggesting that nonantibiotic molecules produ...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2006-08, Vol.103 (33), p.12558-12563
Main Authors: Valle, Jaione, Da Re, Sandra, Henry, Nelly, Fontaine, Thierry, Balestrino, Damien, Latour-Lambert, Patricia, Ghigo, Jean-Marc
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Adhesion
Bacterial Infections - prevention & control
Bacteriology
Biofilms
Biological Sciences
Capsules
Cell Communication - physiology
Colloids
Communities
E coli
Escherichia coli
Escherichia coli - cytology
Escherichia coli - metabolism
Molecules
Pathogens
Polymers
Polysaccharides
Polysaccharides, Bacterial - metabolism
Surface Properties
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Summary:The development of surface-attached biofilm bacterial communities is considered an important source of nosocomial infections. Recently, bacterial interference via signaling molecules and surface active compounds was shown to antagonize biofilm formation, suggesting that nonantibiotic molecules produced during competitive interactions between bacteria could be used for biofilm reduction. Hence, a better understanding of commensal/pathogen interactions within bacterial community could lead to an improved control of exogenous pathogens. To reveal adhesion or growthrelated bacterial interference, we investigated interactions between uropathogenic and commensal Escherichia coli in mixed in vitro biofilms. We demonstrate here that the uropathogenic strain CFT073 and all E. coli expressing group II capsules release into their environment a soluble polysaccharide that induces physicochemical surface alterations, which prevent biofilm formation by a wide range of Gram-positive and Gram-negative bacteria. We show that the treatment of abiotic surfaces with group II capsular polysaccharides drastically reduces both initial adhesion and biofilm development by important nosocomial pathogens. These findings identify capsular polymers as antiadhesion bacterial interference molecules, which may prove to be of significance in the design of new strategies to limit biofilm formation on medical in dwelling devices.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0605399103