Neurogenesis in Dentate Subgranular Zone and Rostral Subventricular Zone after Focal Cerebral Ischemia in the Rat

Because neurogenesis persists in the adult mammalian brain and can be regulated by physiological and pathological events, we investigated its possible involvement in the brain's response to focal cerebral ischemia. Ischemia was induced by occlusion of the middle cerebral artery in the rat for 9...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-04, Vol.98 (8), p.4710-4715
Main Authors: Jin, Kunlin, Minami, Manabu, Lan, Jing Q., Mao, Xiao Ou, Batteur, Sophie, Simon, Roger P., Greenberg, David A.
Format: Article
Language:English
Subjects:
Animals
Behavioral neuroscience
Biological Sciences
Brain
Brain ischemia
Brain Ischemia - metabolism
Brain Ischemia - pathology
Brain Ischemia - physiopathology
Bromodeoxyuridine - metabolism
Cell growth
Cell nucleus
Dentate Gyrus - metabolism
Dentate Gyrus - pathology
Dentate Gyrus - physiopathology
Doublecortin Domain Proteins
Doublecortin Protein
Immunohistochemistry
Ischemia
Male
Microtubule-Associated Proteins
Neural stem cells
Neurogenesis
Neurology
Neurons
Neuropeptides - metabolism
Proliferating Cell Nuclear Antigen - metabolism
Rats
Rats, Sprague-Dawley
Rodents
Stem cells
Stroke
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Summary:Because neurogenesis persists in the adult mammalian brain and can be regulated by physiological and pathological events, we investigated its possible involvement in the brain's response to focal cerebral ischemia. Ischemia was induced by occlusion of the middle cerebral artery in the rat for 90 min, and proliferating cells were labeled with 5-bromo-2′-deoxyuridine-5′-monophosphate (BrdUrd) over 2-day periods before sacrificing animals 1, 2 or 3 weeks after ischemia. Ischemia increased the incorporation of BrdUrd into cells in two neuroproliferative regions-the subgranular zone of the dentate gyrus and the rostral subventricular zone. Both effects were bilateral, but that in the subgranular zone was more prominent on the ischemic side. Cells labeled with BrdUrd coexpressed the immature neuronal markers doublecortin and proliferating cell nuclear antigen but did not express the more mature cell markers NeuN and Hu, suggesting that they were nascent neurons. These results support a role for ischemia-induced neurogenesis in what may be adaptive processes that contribute to recovery after stroke.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.081011098