A High-Resolution Annotated Physical Map of the Human Chromosome 13q12-13 Region Containing the Breast Cancer Susceptibility Locus BRCA2

Various types of physical mapping data were assembled by developing a set of computer programs (Integrated Mapping Package) to derive a detailed, annotated map of a 4-Mb region of human chromosome 13 that includes the BRCA2 locus. The final assembly consists of a yeast artificial chromosome (YAC) co...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-01, Vol.93 (2), p.690-694
Main Authors: Fischer, Stuart G., Cayanis, Eftihia, Maria De Fatima Bonaldo, Bowcock, Anne M., Deaven, Larry L., Edelman, Isidore S., Gallardo, Theresa, Kalachikov, Sergei, Lawton, Lee, Longmire, J. L., Lovett, Michael, Osborne-Lawrence, Sherri, Rothstein, Rodney, Russo, James J., Soares, Marcelo Bento, Sunjevaric, Ivana, Venkatraj, V. S., Warburton, Dorothy, Zhang, Peisen, Efstratiadis, Argiris
Format: Article
Language:English
Subjects:
Base Sequence
BRCA2 Protein
Breast cancer
Breast Neoplasms - genetics
Chromosome Mapping - methods
Chromosomes
Chromosomes, Artificial, Yeast - genetics
Chromosomes, Human, Pair 13 - genetics
Chromosomes, Human, Pair 13 - ultrastructure
Complementary DNA
Computer programming
Cosmids
Cosmids - genetics
Disease Susceptibility
DNA
DNA probes
DNA, Complementary - genetics
Female
Gene Expression
Genetic loci
Genetic mapping
Genetics
Humans
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Neoplasm Proteins - genetics
Oligonucleotide Probes
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
Selection, Genetic
Software
Transcription Factors - genetics
Yeast
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Summary:Various types of physical mapping data were assembled by developing a set of computer programs (Integrated Mapping Package) to derive a detailed, annotated map of a 4-Mb region of human chromosome 13 that includes the BRCA2 locus. The final assembly consists of a yeast artificial chromosome (YAC) contig with 42 members spanning the 13q12-13 region and aligned contigs of 399 cosmids established by cross-hybridization between the cosmids, which were selected from a chromosome 13-specific cosmid library using inter-Alu PCR probes from the YACs. The end sequences of 60 cosmids spaced nearly evenly across the map were used to generate sequence-tagged sites (STSs), which were mapped to the YACs by PCR. A contig framework was generated by STS content mapping, and the map was assembled on this scaffold. Additional annotation was provided by 72 expressed sequences and 10 genetic markers that were positioned on the map by hybridization to cosmids.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.2.690