Intercellular Calcium Signaling Mediated by Point-Source Burst Release of ATP

Calcium signaling, manifested as intercellular waves of rising cytosolic calcium, is, in many cell types, the result of calcium-induced secretion of ATP and activation of purinergic receptors. The mechanism by which ATP is released has hitherto not been established. Here, we show by real-time biolum...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2002-07, Vol.99 (15), p.9840-9845
Main Authors: Arcuino, Gregory, Jane H.-C. Lin, Takano, Takahiro, Liu, Collins, Jiang, Li, Gao, Qun, Kang, Jian, Nedergaard, Maiken
Format: Article
Language:English
Subjects:
Acinar cells
Adenosine Triphosphate - metabolism
Animals
Animals, Newborn
Astrocytes
Astrocytes - cytology
Astrocytes - physiology
Biological Sciences
Calcium
Calcium Signaling - physiology
Cell Communication - physiology
Cell culture techniques
Cells
Cells, Cultured
Cellular biology
Cerebral Cortex - cytology
Endothelial cells
Endothelium, Vascular - physiology
Epithelial cells
Hepatocytes
Human umbilical vein endothelial cells
Humans
Kinetics
Mast cells
Rats
Respiratory Mucosa - cytology
Respiratory Mucosa - physiology
Scientific imaging
Umbilical Veins
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Summary:Calcium signaling, manifested as intercellular waves of rising cytosolic calcium, is, in many cell types, the result of calcium-induced secretion of ATP and activation of purinergic receptors. The mechanism by which ATP is released has hitherto not been established. Here, we show by real-time bioluminescence imaging that ATP efflux is not uniform across a field of cells but is restricted to brief, abrupt point-source bursts. The ATP bursts emanate from single cells and manifest the transient opening of nonselective membrane channels, which admits fluorescent indicators of ≤1.5 kDa. These observations challenge the existence of regenerative ATP release, because ATP efflux is finite and restricted to a point source. Transient efflux of cytosolic nucleotides from a subset of cells may represent a conserved pathway for coordinating local activity of electrically nonexcitable cells, because identical patterns of ATP release were identified in human astrocytes, endothelial cells, and bronchial epithelial cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.152588599