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Optimizing [beta]-carotene production by Blakeslea trispora using bug damaged wheat
Purpose [beta]-carotene, found naturally in many foods, has been widely used as a colourant in various products. The aim of this study is to optimize the production of [beta]-carotene byBlakeslea trispora using bug-damaged wheat as the main nitrogen and carbon source. Design/methodology/approach Res...
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Published in: | Pigment & resin technology 2018-05, Vol.47 (3), p.189 |
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Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Purpose [beta]-carotene, found naturally in many foods, has been widely used as a colourant in various products. The aim of this study is to optimize the production of [beta]-carotene byBlakeslea trispora using bug-damaged wheat as the main nitrogen and carbon source. Design/methodology/approach Response surface methodology (RSM) has been used to determine the optimum concentrations of media components for the maximum [beta]-carotene production. [beta]-carotene concentration was determined quantitively using a spectrophotometric method. Findings A full quadratic model fit the data best for [beta]-carotene production with a highR 2 value (0.999). Using optimized media increased the production of [beta]-carotene byB. trispora DSM-2387 andB. trispora DSM-2388 to a higher level (250.407 mg/l) than the control medium (72.195 mg/l). Originality/value This study showed that bug-damaged wheat, an agro-industrial waste having low economical value, may be used as a valuable substrate for microbial production of [beta]-carotene, a natural food colourant possessing high economical value. |
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ISSN: | 0369-9420 1758-6941 |