Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

Induced pluripotent stem (iPS) cells represent a powerful source for cell-based tissue regeneration because they are patient-specific cells and can differentiate into specialized cell types. Previously, we have demonstrated the derivation of neural crest like cells from iPS cells (iPS-NCLCs), and th...

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Bibliographic Details
Published in:Journal of Hard Tissue Biology 2018/01/01, Vol.27(1), pp.1-10
Main Authors: Kikuchi, Kazuko, Masuda, Tomoyuki, Fujiwara, Naoki, Kuji, Akiyoshi, Miura, Hiroyuki, Jung, Han-Sung, Harada, Hidemitsu, Otsu, Keishi
Format: Article
Language:English
Subjects:
Adipocytes
Biocompatibility
Biomarkers
Biomedical materials
Bone growth
Chondrocytes
Craniofacial bone regeneration
Dental pulp
Hydroxyapatite
Immunodeficiency
Induced pluripotent stem cells (iPSCs)
Mesenchymal stem cells
Mesenchyme
Mice
Neural crest
Neural crest cells
Odontoblasts
Osteoblasts
Pluripotency
Regeneration
Regeneration (physiology)
Stem cell transplantation
Stem cells
Surgical implants
Tissue engineering
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Summary:Induced pluripotent stem (iPS) cells represent a powerful source for cell-based tissue regeneration because they are patient-specific cells and can differentiate into specialized cell types. Previously, we have demonstrated the derivation of neural crest like cells from iPS cells (iPS-NCLCs), and these cells have the potential to differentiate into dental mesenchymal cells, which subsequently differentiate into odontoblasts and dental pulp cells. In this study, we show that iPS-NCLCs can differentiate into mesenchymal stem cells (iPS-NCLC-MSCs), which contribute to craniofacial bone regeneration. iPS-NCLCs were cultured in serum-containing media and differentiated into functional MSCs, as confirmed by expression MSC markers and their ability to differentiate into osteoblasts, adipocytes, and chondrocytes in vitro. iPS-NCLC-MSCs were negative for markers of undifferentiated iPS cells and did not develop into teratomas when transplanted to immunodeficient mice. Further, iPS-NCLC-MSCs grew normally and differentiated into osteoblasts on hydroxyapatite scaffolds in vitro. To assess the potential of iPS-NCLC-MSCs to regenerate craniofacial bone in vivo, iPS-NCLC-MSCs were transplanted into critical-size calvarial defects in immunodeficient mice for 8 weeks. Histological analysis revealed that iPS-NCLC-MSCs differentiated into osteoblasts and contributed to bone regeneration without tumor formation. These results indicate that iPS-NCLC-MSCs could be a potential candidate for cell-based craniofacial bone tissue repair and regeneration.
ISSN:1341-7649
1880-828X
DOI:10.2485/jhtb.27.1