Antibacterial Activity and Biocompability of Zinc Oxide and Graphite Particles as Endodontic Materials

The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus...

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Bibliographic Details
Published in:Journal of Hard Tissue Biology 2017/10/01, Vol.26(4), pp.311-318
Main Authors: Kozuszko, Silvia Noemí, Sánchez, María Alejandra, María Inés Gutiérrez de Ferro, Sfer, Ana María, Madrid, Ana Paula Moreno, Takabatake, Kiyofumi, Nakano, Keisuke, Nagatsuka, Hitoshi, Rodríguez, Andrea Paola
Format: Article
Language:English
Subjects:
Antibacterial activity
Antibacterial Agents
Biocompatibility
Calcium
Calcium hydroxide
Collagen
Connective tissues
Cytoplasm
Foreign body reaction
Giant cells
Graphite
Inflammation
Microorganisms
Microparticles
Particulates
Powder
Rats
Regeneration
Slaked lime
Staphylococcus aureus
Tissue engineering
Wound healing
Zinc
Zinc Oxide
Zinc oxides
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Summary:The aim of this work is to evaluate the antibacterial effect and biocompatibility of zinc oxide (ZnO) nanopaticles and graphite-type carbon (Gt) microparticles commercial powders. SEM analysis was performed to assess particles morphology. The antibacterial behavior was studied against Staphylococcus aureus (Staph. Aureus) (bacterial strain ATCC 29213) and TEM analysis of bacteria was performed to determine ultrastructural alterations; in addition, biocompatibility was evaluated in subcutaneous tissue of Wistar rats at 3, 7 and 28 d. ZnO and Gt powders exhibited antibacterial activity while TEM images of Staph. aureus showed membrane disruption followed by the release of internal content. Also, an electron-light region within the cytoplasm was observed for microorganisms treated with ZnO. Regarding biocompatibility, Gt samples induced a foreign body reaction response with presence of giant cells whereas ZnO samples showed fibroblastic connective tissue with chronic inflammatory cells and new small vessels. Also, collagen fibers and lack of capsule was observed by Trichome Masson stain. Thus, ZnO improved wound healing by enhancing tissue regeneration in contrast with calcium hydroxide control sample response which showed a fibrous tissue scar. Hence, ZnO nano-powder seems to be a potential material in the regenerative endodontic field.
ISSN:1341-7649
1880-828X
DOI:10.2485/jhtb.26.311