Acrylamide and Acrylic Acid Biodegradation by Alcaligenes faecalis 2 Planktonic Cells and Biofilms

• Published in: Applied biochemistry and microbiology 2018-03, Vol.54 (2), p.173-178
• Main Authors: Maksimova, Yu. G., Vasil’ev, D. M., Zorina, A. S., Ovechkina, G. V., Maksimov, A. Yu
• Format: Article
• Language: English
• Subjects: Acids; Acrylamide; Acrylic acid; Acrylics; Alcaligenes; Alcaligenes faecalis; Basalt; Biochemistry; Biodegradation; Biofilms; Biomedical and Life Sciences; Carbon sources; Cultivation; Dry cells; Life Sciences; Medical Microbiology; Microbiology; Planktonic cells
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683818020084

The ability of the Alcaligenes faecalis 2 strain to utilize acrylamide and acrylic acid upon cultivation with these compounds as the only sources of carbon and energy has been investigated. Complete utilization of the acrylic acid present in the medium at concentrations below 0.113 g/L was observed by cultivation day 5, at a concentration of 0.225 g/L by day 7, and at a concentration of 0.45 g/L by day 17. Complete utilization of the acrylamide present in the medium at concentrations below 0.4 g/L was observed by day 5, at a concentration of 0.9 g/L by day 7, and at a concentration of 1.8 g/L by day 20. Importantly, bacterial growth did not start before complete transformation of acrylamide into acrylic acid. The rate of acrylamide transformation by growing bacteria and a cell suspension in the stationary growth phase amounted to 12.5 mg/L h at a cell concentration of 610 mg/L and 300 mg/L h, at a concentration of 1500 mg/L. A. faecalis 2 cells immobilized on BVV-22 basalt fibers and Carbopon-B-aktiv at concentrations of 3000 and 800 mg dry cells/L, respectively, transformed acrylamide at a rate of 1200 mg/L h.