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Enhancement and characterization of natural pigments produced by Monascus spp. using Saccharina japonica as fermentation substrate
The study aimed to maximize the production of natural pigments from Monascus spp. using Saccharina japonica as a solid state fermentation substrate at optimized physical and nutritional conditions. The yield of yellow, orange, and red pigments of Monascus purpureus were 83.01, 80.07, and 79.87 AU g...
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Published in: | Journal of applied phycology 2018-02, Vol.30 (1), p.729-742 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The study aimed to maximize the production of natural pigments from
Monascus
spp. using
Saccharina japonica
as a solid state fermentation substrate at optimized physical and nutritional conditions. The yield of yellow, orange, and red pigments of
Monascus purpureus
were 83.01, 80.07, and 79.87 AU g
dfs
−1
and
Monascus kaoliang
were 83.23, 77.22, and 75.09 AU g
dfs
−1
, respectively, when providing external 0.8% glucose and 0.4% peptone at incubation temperature 30 °C for 20 days. Medium inoculum size of 3 mL and moisture content of 50% for
M. purpureus
and 60% for
M. kaoliang
showed the highest pigment production. Scanning electron microscopy showed better mycelial growth of
M. purpureus
than
M. kaoliang
on
S. japonica
.
Monascus
spp. fermented seaweed was highly bioactive showing DNA protection and antioxidant activity because of increasing phenolic and flavonoid contents during fermentation period. The highest reducing sugar content was found at 15 days and glucanase enzyme activity was found at 20 days of fermentation for both of the species. The pigments were separated by thin layer of chromatography and the
R
f
values were obtained 0.91 and 0.84 for yellow and orange pigments and two spots of 0.69 and 0.41 for red pigments. At different physicochemical stress, red pigment of
M. purpureus
showed more stability than that of
M. kaoliang
. So, it can be concluded that
S. japonica
was a very suitable substrate at optimum conditions for
Monascus
spp. natural pigment production. |
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ISSN: | 0921-8971 1573-5176 |
DOI: | 10.1007/s10811-017-1258-4 |