Nitrogen uptake and growth responses of seedlings of the brown seaweed Sargassum hemiphyllum under controlled culture conditions

For the purpose of indoor mass culture of seedlings of Sargassum hemiphyllum during spring and summer, a series of experiments were designed to evaluate nitrogen (N) uptake, growth performance, and N accumulation of seedlings. Transient uptake rates by N-limited seedlings for nitrate (NO 3 − ), ammo...

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Bibliographic Details
Published in:Journal of applied phycology 2018-02, Vol.30 (1), p.507-515
Main Authors: Han, Tingting, Qi, Zhanhui, Huang, Honghui, Liao, Xiuli, Zhang, Wenwen
Format: Article
Language:English
Subjects:
Algae
Ammonium
Ammonium compounds
Availability
Biomedical and Life Sciences
Dry weight
Ecology
Freshwater & Marine Ecology
Growth
Life Sciences
Mass culture
Nitrates
Nitrogen
Plant Physiology
Plant Sciences
Sargassum
Seaweeds
Seedlings
Tissue
Uptake
Urea
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Summary:For the purpose of indoor mass culture of seedlings of Sargassum hemiphyllum during spring and summer, a series of experiments were designed to evaluate nitrogen (N) uptake, growth performance, and N accumulation of seedlings. Transient uptake rates by N-limited seedlings for nitrate (NO 3 − ), ammonium (NH 4 + ), and urea were more rapid than uptake rates by N-replete seedlings. NO 3 − uptake revealed a rate-saturating uptake kinetic for both N-limited and N-replete seedlings. Uptake rates of NH 4 + and urea for N-limited seedlings were linear, whereas the N-replete seedlings displayed Michaelis-Menten curves. The slope of the linear models or equivalently affinity calculated from Michaelis-Menten curves was highest for NH 4 + and lowest for urea, indicating that seedlings were more efficient at taking up NH 4 + . Tissue N content in seedling increased with increasing N concentrations, with the highest values with all three N sources at 150 μM. The N requirement to support maximum growth varied 2.2-fold from 0.39 ± 0.15% dry weight (DW) in NH 4 + to 0.84 ± 0.09% DW in NO 3 − , and hence, seedlings became more easily N limited at low NO 3 − availability. The results showed that seedlings cultured in NO 3 − enrichment (50–100 μM) may be better able to meet their maximum growth than at high NH 4 + availability, although seedlings have higher NH 4 + uptake capacities.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-017-1216-1