Light-induced Wilting and its Molecular Mechanism in Epicotyls of Dark-germinated Pea (Pisum sativum L.) Seedlings

Possible mechanisms behind the light-induced wilting of dark-germinated pea (Pisum sativum L.) epicotyls were studied. Illumination with photosynthetically active radiation caused a fast turgor loss and wilting in the middle segments of the epicotyls accompanied by accumulation of water in the inter...

Full description

Saved in:
Bibliographic Details
Published in:Plant and cell physiology 2005-01, Vol.46 (1), p.185-191
Main Authors: Erdei, Noémi, Barta, Csengele, Hideg, Éva, Böddi, Béla
Format: Article
Language:English
Subjects:
3-[N-(β-diethylaminoethyl)-N-dansyl]aminomethyl-2
3-tetraethoxypropane
5-dihydro-1H-pyrrole
5-tetramethyl-2
Chlide
chlorophyllide
DanePy
Germination
Keywords: Chlorophyllous pigments — Lipid peroxidation — Pea epicotyl — Photooxidative — Protochlorophyllide — Singlet oxygen
Light
Lipid Peroxidation - radiation effects
malondialdehyde
MDA
PAR
Pchl
Pchlide
Photobiology
photosynthetically active radiation
Pisum sativum - growth & development
Pisum sativum - metabolism
Pisum sativum - radiation effects
PLB
Porphyrins - metabolism
Porphyrins - radiation effects
prolamellar body
protochlorophyllide
protochlorophyllide ester
reactive oxygen species
ROS
Seedlings - growth & development
Seedlings - metabolism
Seedlings - radiation effects
Singlet Oxygen - metabolism
Spectrometry, Fluorescence
TBA
TBARS
TEP
thiobarbituric acid
thiobarbituric acid-reactive substances
Water - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Possible mechanisms behind the light-induced wilting of dark-germinated pea (Pisum sativum L.) epicotyls were studied. Illumination with photosynthetically active radiation caused a fast turgor loss and wilting in the middle segments of the epicotyls accompanied by accumulation of water in the intercellular cavities. During this process, room temperature fluorescence emission spectra showed gradual bleaching of porphyrin-type pigments, which was lessened by incubating the epicotyls with excess ascorbate before illumination. Detection of singlet oxygen and lipid peroxidation products in the illuminated epicotyls suggested the occurrence of porphyrin-photosenzitized membrane damage as a cause of disordered water status and sequential wilting.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pci012