Association of glutathione with flowering in Arabidopsis thaliana

In order to study the relatipnship between GSH and flowering, wild-type and late-flowering mutant, fca-1, of Arabidopsis thaliana were treated with L-buthionine sulfoximine (BSO), a specific inhibitor of GSH biosynthesis, under long-day conditions. BSO treatment of the fca-1 mutant starting at 17 d...

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Bibliographic Details
Published in:Plant and cell physiology 2001-05, Vol.42 (5), p.524
Main Authors: Ogawa, K. (Okayama-ken. Research Inst. for Biological Sciences, Kayo (Japan)), Tasaka, Y, Mino, M, Tanaka, Y, Iwabuchi, M
Format: Article
Language:English
Subjects:
Arabidopsis - enzymology
Arabidopsis - growth & development
Arabidopsis - physiology
ARABIDOPSIS THALIANA
BIOSYNTHESIS
Buthionine Sulfoximine
Cyclophosphamide - antagonists & inhibitors
FLOWERING
GLUTATHIONE
Glutathione - metabolism
Glutathione - physiology
Glutathione Transferase - metabolism
Light
Methionine Sulfoximine - analogs & derivatives
Methionine Sulfoximine - pharmacology
Morphogenesis
MUTANTS
Mutation
Phenotype
PHOTOPERIODICITY
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Summary:In order to study the relatipnship between GSH and flowering, wild-type and late-flowering mutant, fca-1, of Arabidopsis thaliana were treated with L-buthionine sulfoximine (BSO), a specific inhibitor of GSH biosynthesis, under long-day conditions. BSO treatment of the fca-1 mutant starting at 17 d after imbibition promoted flowering. However, when the treatment was started at 12 d after imbibition, BSO treatment at 10E-4 M resulted in an inhibition of flowering. This inhibitory effect of BSO on flowering was abolished by GSH treatment at 10E-4 M, although GSH treatment at an increased concentration of 10E-3 M clearly delayed flowering. In contrast, BSO treatment of wild-type plants starting at, 12 d after imbibition promoted flowering, whose effect was abolished by GSH application. In the fca-1 mutant, whose endogenous GSH levels were high, chilling treatment lowered tbe GSH levels and promoted flowering, as was the case in the BSO treatment. An A. thaliana mutant, cad2-1, which has a defect in GSH biosynthesis also exhibited late flowering. The late-flowering phenotype of this mutant tended to be strengthened by BSO and abolished by GSH treatment. These results suggest that flowering is associated with the rate of GSH biosynthesis and / or the levels of GSH in A. thaliana.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pce065