New Design Micro Total Analysis System for Determination Hydrazine in Pharmaceutical Samples

• Published in: Journal of pharmaceutical sciences and research 2018-02, Vol.10 (2), p.248-253
• Main Authors: Mashkoor, Muthana Saleh, Al-Khafagi, Ahmed Adnan, Taha, Dakhil Nassir
• Format: Article
• Language: English
• Subjects: Chemistry; Design; Pharmaceuticals; Studies; Sulfuric acid
• ISSN: 0975-1459

2.Experimental 2.1.Apparatus Spectrophotometer Labomed.inG single beam, Analytical balance sensitive Denver Instrument, USA, and a spectrophotometer Shimadzu UV-1700 spectrophotometer, peristaltic pump Germany, Ismatic, Recorder Pen Siemens C 1032,files Interaction with the radius of 0.5 mm, homemade microfluidic chip , pipes load of Teflon, flow cell volume of 450 pL. 2.2 Unit The various condition affecting the unit have been investigated and selected for a final method evaluation; the following results allow the operator to choose different operation conditions, many new designs of chips were developed by the researchers [16,26]. The result showed that the maximum absorbance wavelength of the yellow compound is 410 nm, whereas the reagent shows maximum absorbance at 363nm. [...]410nm was chosen as optimum wavelength for further studies. 3.2.Study of optimum conditions 3.2.1. Effect of Reaction Coil Length Study the effect of the Reaction Coil Length within the range (50100) cm, and that the conditions of the system are Concentration of vanillin is 0.026 M Concentration of hydrazine sulfate is 4.611 x 10- 4 M, Concentration of sulfuric acid is 0.2 M ,Volume of the sample is 235.650 pL loaded in the loop of sample (Ls), Flow rate for the three of carriers stream is 14.80 ml.min-1 ,The length of the reaction coil is (50 - 100 ) cm .Results of the study showed that signal increases with the Reaction Coil Length until access to the 75 cm then begins to decline and thus the optimal Reaction Coil Length is to be 75 cm, as in the table ( 1) and figure ( 5 ) illustrates the results 3.2.1.2. Effect of Flow Rate Study the effect of the flow rate within the range (3.30-6.60) ml / min, and that the conditions of the system are Concentration of vanillin is 0.026 M , Concentration of hydrazine sulfate is 4.611 x 10- 4 M, Concentration of sulfuric acid is 0.2 M , Volume of the sample is 235.650 pL loaded in the loop of sample (Ls), Flow rate for the three of carriers stream is (3.30-6.60) ml.min-1 , The length of the reaction coil is 75 cm .Results of the study showed that the absorbance increased with increasing flow rate until access to the flow rate4.90 ml.min.-1 then begins to decline, and this is the optimal flow rate is 4.90 ml.min.-1, as in the table (2) and figure(6) illustrates the results 3.2.1.3.