Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model

Exposure of the skin to ultraviolet (UV) radiation causes extracellular matrix (ECM) collapse in the dermis, owing to an increase in matrix metalloproteinase (MMP) production in both the epidermis and dermis, and a decrease in type I collagen expression in the dermis. Recently, black rice (Oryza sat...

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Bibliographic Details
Published in:International journal of molecular medicine 2018-05, Vol.41 (5), p.3073-3080
Main Authors: Han, Mira, Bae, Jung-Soo, Ban, Jae-Jun, Shin, Hee Soon, Lee, Dong Hun, Ghung, Jin Ho
Format: Article
Language:English
Subjects:
Antioxidants
Binding sites
Cancer
Collagen
Enzyme regulation
Flavonoids
Health aspects
Kinases
Properties
Proteases
Proteins
Reactive oxygen species
Rice
Skin
Ultraviolet radiation
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Summary:Exposure of the skin to ultraviolet (UV) radiation causes extracellular matrix (ECM) collapse in the dermis, owing to an increase in matrix metalloproteinase (MMP) production in both the epidermis and dermis, and a decrease in type I collagen expression in the dermis. Recently, black rice (Oryza sativa L.) was reported to have a wide range of pharmacological effects in various settings. However, the effects of black rice extract (BRE) on UV-irradiated skin cells have not yet been characterized. BRE treatment did not affect cell morphology and viability of HaCaT and human dermal fibroblasts (HDF). We demonstrated that BRE downregulated basal and UV-induced MMP-1 expression in HacaT cells. Furthermore, BRE significantly increased type I procollagen expression, and decreased MMP-1 and MMP-3 expression in UV-irradiated HDF. The underlying mechanisms of these results involve a decrease in p38 and c-Jun N-terminal kinase activity, and suppression of UV-induced activation of activator protein-1 (AP-1). BRE reduced UV-induced reactive oxygen species production in HacaT cells in a dose-dependent manner. Indeed, mass spectrometry revealed that BRE contained antioxidative flavonoid components such as cyanidin-3-O-[beta]-D-glycoside and taxifolin-7-O-glucoside. These findings suggest that BRE attenuates UV-induced ECM damage by modulating mitogen-activated protein kinase and AP-1 signaling, and could be used as an active ingredient for preventing photoaging of the skin. Key words: black rice, matrix metalloproteinases, type I procollagen, reactive oxygen species, activator protein-1, mitogen-activated protein kinase
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2018.3508