Development of docetaxel-loaded PEG–PLA nanoparticles using surfactant-free method for controlled release studies

Docetaxel (DTX)-loaded poly(ethylene glycol)-poly(D,L-lactide) is prepared by nanoprecipitation method in the absence of any surfactants. The average particle size of the copolymer was found to be 101 nm. The drug entrapment efficiency (%) and drug loading (%) of polymer were found to be 9.471 ± 0.0...

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Bibliographic Details
Published in:International journal of polymeric materials 2018-06, Vol.67 (9), p.535-542
Main Authors: Mishra, Prajna, Dey, Ratan Kumar
Format: Article
Language:English
Subjects:
Apoptosis
Controlled release
Drug delivery systems
Entrapment
Polyethylene glycol
Ribose
Surfactants
Toxicity
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Summary:Docetaxel (DTX)-loaded poly(ethylene glycol)-poly(D,L-lactide) is prepared by nanoprecipitation method in the absence of any surfactants. The average particle size of the copolymer was found to be 101 nm. The drug entrapment efficiency (%) and drug loading (%) of polymer were found to be 9.471 ± 0.047 and 94.71 ± 0.466, respectively. The in vitro drug release characteristics show the controlled release of 98% of docetaxel in 72 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and apoptosis measured in terms of cleaved Poly(ADP-ribose) Polymerase (PARP) and cleaved caspase-3 protein expression shows that the copolymer has better cytotoxicity effect and apoptosis in comparison to free DTX in HeLa cells.
ISSN:0091-4037
1563-535X
DOI:10.1080/00914037.2017.1354193