Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion

We present an investigation of horseradish peroxidase (HRP)/H 2 O 2 -mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with ami...

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Bibliographic Details
Published in:Colloid and polymer science 2018-06, Vol.296 (6), p.995-1003
Main Authors: Petr, Šálek, Jana, Dvořáková, Peter, Černoch, Ewa, Pavlova, Vladimír, Proks
Format: Article
Language:English
Subjects:
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Complex Fluids and Microfluidics
Crosslinking
Cyclohexane
Food Science
Hydrogen peroxide
Morphology
Nanoparticles
Nanotechnology and Microengineering
Original Contribution
Peroxidase
Photon correlation spectroscopy
Physical Chemistry
Polymer Sciences
Polymers
Prepolymers
Soft and Granular Matter
Transmission electron microscopy
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Summary:We present an investigation of horseradish peroxidase (HRP)/H 2 O 2 -mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β- N -(2-hydroxyethyl)-D,L-aspartamide- co - N- (2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii ( R h 0 ), mean size documented by hydrodynamic radius ( R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor.
ISSN:0303-402X
1435-1536
DOI:10.1007/s00396-018-4318-7