Raspberry ketone protects against isoproterenol-induced myocardial infarction in rats

• Published in: Life sciences (1973) 2018-02, Vol.194, p.205-212
• Main Authors: Khan, Vasim, Sharma, Sumit, Bhandari, Uma, Ali, Syed Mansoor, Haque, Syed Ehtaishamul
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - therapeutic use; Antioxidants - therapeutic use; Berries; Butanones - chemistry; Butanones - therapeutic use; Cardiotonic Agents - therapeutic use; Catalase; Cholesterol; Creatine; Creatine kinase; Density; Dyslipidemia; Glutathione; Heart attacks; Immunohistochemistry; Inflammation; Isoproterenol; Ketones; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Malondialdehyde; Myocardial infarction; Myocardial Infarction - chemically induced; Myocardial Infarction - pathology; Myocardial Infarction - prevention & control; Myocardium - pathology; Na+/K+-exchanging ATPase; Nitric oxide; Nitric-oxide synthase; Organic chemicals; Oxidative stress; Oxidative Stress - drug effects; PPAR alpha - agonists; Prescription drugs; Propranolol; Raspberry ketone; Rats; Rats, Wistar; Rodents; Rubus - chemistry; Rubus idaeus; Superoxide dismutase; Triglycerides; Tumor necrosis factor-α
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2017.12.013

The cardioprotective role of raspberry ketone (RK) against isoproterenol (ISO)-induced myocardial infarction (MI) in rats was assessed. Rats were randomly divided into Group I - Vehicle control; Group II - Toxic control ISO (85mg/kg, s.c.); Group III, IV and V - RK (50, 100 and 200mg/kg, respectively) with ISO; Group VI- RK (200mg/kg) alone; Group VII - Propranolol (10mg/kg) with ISO; and Group VIII - Propranolol (10mg/kg) alone. After twenty-four hours of the last dose, animals were sacrificed and creatine kinase-MB, lactate dehydrogenase, total cholesterol, triglycerides, high-density-lipoprotein, low-density-lipoprotein, very-low-density-lipoprotein, malondialdehyde, reduced glutathione, superoxide dismutase, catalase, Na+, K+-ATPase, nitric oxide, histopathological and immunohistochemical analysis (tumor necrosis factor-α and inducible nitric oxide synthase) were performed. Treatment with ISO significantly deviated the biochemical parameters from the normal levels, which were considerably restored by RK at 100 and 200mg/kg doses. 50mg/kg dose, however, did not demonstrate any significant cardioprotective action. The histopathological and immunohistochemical analysis further substantiated these findings. Our study showed a dose-dependent reduction in oxidative stress, inflammation and dyslipidemia by RK in ISO-intoxicated rats, which signifies that RK from the European red raspberry plant might be a valuable entity for the management of MI. [Display omitted] •The cardioprotective action of raspberry ketone in isoproterenol induced cardiotoxicity was assessed.•The cardiac damage due to isoproterenol was significantly prevented by prophylactic treatment of raspberry ketone.•The antioxidative, antihyperlipidemic, and anti-inflammatory properties of raspberry ketone plays role in cardioprotection.•The protective effects of raspberry ketone were comparable to that of standard β-blocking drug, propranolol.•The protective effects of raspberry ketone might be attributed to its PPAR-α agonistic activity.