miR-137 modulates coelomocyte apoptosis by targeting 14-3-3? in the sea cucumber Apostichopus japonicus

MicroRNAs (miRNAs) have emerged as key regulators in the host immune response and play a pivotal role in host-pathogen interactions by suppressing the transcriptional and post-transcriptional expression of target genes. miR-137, a well-documented tumor repressor, was previously found by high-through...

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Bibliographic Details
Published in:Developmental and comparative immunology 2017-02, Vol.67, p.86
Main Authors: Lv, Miao, Chen, Huahui, Shao, Yina, Li, Chenghua, Xu, Wei, Zhang, Weiwei, Zhao, Xuelin, Duan, Xuemei
Format: Article
Language:English
Subjects:
14-3-3 protein
Apoptosis
Aquatic life
Bacteria
Coelomocytes
Cytometry
Flow cytometry
Gene expression
Gene sequencing
Genes
Host-pathogen interactions
Immune response
Immune system
Inhibition
Lipopolysaccharides
miRNA
Next-generation sequencing
Pathogenesis
Post-transcription
Regulators
siRNA
Waterborne diseases
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Summary:MicroRNAs (miRNAs) have emerged as key regulators in the host immune response and play a pivotal role in host-pathogen interactions by suppressing the transcriptional and post-transcriptional expression of target genes. miR-137, a well-documented tumor repressor, was previously found by high-throughput sequencing to be differentially expressed in diseased specimens of the sea cucumber Apostichopus japonicas. In this study, we identified 14-3-3ζ protein (Aj14-3-3ζ) as a novel target of miR-137 using isobaric tags for relative and absolute quantification (iTRAQ) and transcriptome screening. Expression analysis indicated that consistently depressed expression profiles of miR-137 and Aj14-3-3ζ were detected in both LPS-exposed primary coelomocytes and Vibrio splendidus-challenged sea cucumbers, suggesting a positive regulatory interaction. Consistently, miR-137 overexpression or inhibition in vitro and in vivo showed no effect on Aj14-3-3ζ mRNA levels, but the concentration of Aj14-3-3ζ protein was induced or repressed, respectively. Moreover, siRNA-mediated Aj14-3-3ζ knockdown in vivo decreased both mRNA and protein expression levels of Aj14-3-3ζ and significantly promoted coelomocyte apoptosis as assessed by flow cytometry, consistent with miR-137 inhibition. Overall, these results enhance our understanding of miR-137 regulatory roles in sea cucumber pathogenesis.
ISSN:0145-305X
1879-0089