The detection of active moulds on historical silk by the means of the headspace–solid phase micro-extraction–gas chromatography–mass spectrometry method

The goal of this work was to analyze the profile of microbial volatile organic compounds (MVOCs) emitted by moulds growing on silk samples in search of particular volatiles mentioned in the literature as indicators of active mould growth. The selected moulds were inoculated on three types of media:...

Full description

Saved in:
Bibliographic Details
Published in:Textile research journal 2018-05, Vol.88 (9), p.1013-1025
Main Authors: Sawoszczuk, Tomasz, Syguła-Cholewińska, Justyna, del Hoyo-Meléndez, Julio M
Format: Article
Language:English
Subjects:
Amino acids
Authorship
Carbon sources
Chromatography
Cultural heritage
Decision making
Divinylbenzene
Fibers
Fungi
Gas chromatography
Headspace
Humidity
Indicators
Investigations
Mass spectrometry
Mass spectroscopy
Materials research
Microorganisms
Mold
Mold growths
Museums
Object recognition
Open systems
Organic carbon
Organic compounds
Polydimethylsiloxane
Qualitative analysis
Scientific imaging
Silicone resins
Silk
Spectroscopy
Sulfur
Textiles
VOCs
Volatile compounds
Volatile organic compounds
Volatiles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The goal of this work was to analyze the profile of microbial volatile organic compounds (MVOCs) emitted by moulds growing on silk samples in search of particular volatiles mentioned in the literature as indicators of active mould growth. The selected moulds were inoculated on three types of media: (1) samples of pure silk placed on microbial media, (2) samples of historical silk placed on microbial media, and (3) agar containing amino acids that are elements of the structure of fibroin. All samples were prepared inside vials (closed system). In the first and second cases the media did not contain any sources of organic carbon, nitrogen, or sulfur—the silk was the only nutrient for the moulds. A fourth type of sample was historical silk prepared in a Petri dish without a microbial medium and inoculated with a fibroinolytically active mould (open system). The MVOCs emitted by moulds were sampled with the headspace–solid phase micro-extraction method. Volatiles extracted on Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS) fibers were analyzed in a gas chromatography–mass spectrometry system. Qualitative and quantitative analyses of chromatograms were carried out in search of indicators of metabolic activity. The results showed that there are three groups of volatiles that can be used for the detection of active forms of moulds on silk. Thus, this can be a valuable tool for the fast detection of mould activity on historical silk objects. To the best of our knowledge, this is the first work to measure MVOCs emitted by moulds growing on silk.
ISSN:0040-5175
1746-7748
DOI:10.1177/0040517517693984