Brassinosteroid‐mediated apoplastic H2O2‐glutaredoxin 12/14 cascade regulates antioxidant capacity in response to chilling in tomato

Brassinosteroids (BRs) regulate plant development and stress response. Although much has been learned about their roles in plant development, the mechanisms by which BRs regulate plant stress tolerance remain unclear. Chilling is a major stress that adversely affects plant growth. Here, we report th...

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Published in:Plant, cell and environment cell and environment, 2018-05, Vol.41 (5), p.1052-1064
Main Authors: Xia, Xiao‐Jian, Fang, Ping‐Ping, Guo, Xie, Qian, Xiang‐Jie, Zhou, Jie, Shi, Kai, Zhou, Yan‐Hong, Yu, Jing‐Quan
Format: Article
Language:English
Subjects:
2‐cysteine peroxiredoxin
Antioxidants
Brassinosteroids
Cellular stress response
Chilling
Cooling
Enzymes
Genes
Glutaredoxin
glutaredoxins
Homology
Hydrogen peroxide
Lipid peroxidation
Membrane proteins
NADPH oxidase
Peroxidation
Peroxiredoxin
Photosystem II
Plant growth
Plant stress
Quantum efficiency
reactive oxygen species
Respiratory burst oxidase
RNA-mediated interference
Signaling
Solanum lycopersicum
Tomatoes
Transcription activation
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Summary:Brassinosteroids (BRs) regulate plant development and stress response. Although much has been learned about their roles in plant development, the mechanisms by which BRs regulate plant stress tolerance remain unclear. Chilling is a major stress that adversely affects plant growth. Here, we report that BR positively regulates chilling tolerance in tomato. BR partial deficiency aggravated chilling‐induced oxidized protein accumulation, membrane lipid peroxidation, and decrease of maximum quantum efficiency of photosystem II (Fv/Fm). By contrast, overexpression of BR biosynthetic gene Dwarf or treatment with 24‐epibrassinolide (EBR) attenuated chilling‐induced oxidative damages and resulted in an increase of Fv/Fm. BR increased transcripts of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1) and GLUTAREDOXIN (GRX) genes, and BR‐induced chilling tolerance was associated with an increase in the ratio of reduced/oxidized 2‐cysteine peroxiredoxin (2‐Cys Prx) and activation of antioxidant enzymes. However, RBOH1‐RNAi plants failed to respond to EBR as regards to the induction of GRX genes, activation of antioxidant capacity, and attenuation of chilling‐induced oxidative damages. Furthermore, silencing of GRXS12 and S14 compromised EBR‐induced increases in the ratio of reduced/oxidized 2‐Cys Prx and activities of antioxidant enzymes. Our study suggests that BR enhances chilling tolerance through a signalling cascade involving RBOH1, GRXs, and 2‐Cys Prx in tomato. BRs confer plant tolerance to a range of stresses, whereas the mechanisms underlie remain unclear. Plant endogenous BR levels positively regulate chilling tolerance in tomato. BR‐induced chilling tolerance is well associated with an increase in the ratio of 2‐Cys peroxiredoxin monomer to dimer and enhancement of activities of antioxidant enzymes. The apoplastic H2O2‐glutaredoxin 12/14 signalling cascade is involved in BR‐induced chilling tolerance.
ISSN:0140-7791
1365-3040
DOI:10.1111/pce.13052