Survey of Citrus tristeza virus (CTV) diversity in pigmented Citrus x paradisi (Macfad.) (Grapefruit) trees in north-western Argentina

Citrus tristeza virus (CTV) is the most severe viral pathogen of citrus and elicits a wide range of devastating disease symptoms. Grapefruit cultivars ( Citrus x paradisi ) are the most sensitive among citrus to the effects of CTV infections. Grapefruit is an important crop within the north-western...

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Bibliographic Details
Published in:European journal of plant pathology 2018-06, Vol.151 (2), p.329-340
Main Authors: Read, D. A., Palacios, M. F., Kleynhans, J., Figueroa, J., Foguet, L., Stein, B., Pietersen, G.
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Citrus fruits
Citrus paradisi
Closterovirus
Clustering
Cultivars
Cultivated plants
Ecology
Fruit trees
Fruits
Gene sequencing
Grapefruit
Life Sciences
Plant Pathology
Plant Sciences
Plant virus diseases
Plant viruses
Populations
Viruses
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Summary:Citrus tristeza virus (CTV) is the most severe viral pathogen of citrus and elicits a wide range of devastating disease symptoms. Grapefruit cultivars ( Citrus x paradisi ) are the most sensitive among citrus to the effects of CTV infections. Grapefruit is an important crop within the north-western Argentine citrus industry; however, production has been affected by CTV stem-pitting. In general, CTV diversity within South America is poorly studied, with data on grapefruit CTV populations being particularly limited. In this study, 50 samples were collected from Star Ruby, Henninger’s Ruby and Ruben Pink cultivars, within the provinces of Tucumán, Salta and Jujuy in north-western Argentina. The CTV p33 gene was PCR amplified and the resulting amplicons sequenced with Sanger sequencing. A subset of these amplicons was sequenced with Illumina MiSeq sequencing. AT-1-like sequences were dominant within the majority of populations, as determined by Sanger sequencing, followed by sequences clustering within the unresolved Kpg3/SP/T3 and RB clades. Sequencing by Illumina MiSeq confirmed this, as well as detecting minor sequence types within the HA 16–5, VT, B165 and A18 clades.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-017-1376-5