Storage strategies and processing of bovine milk samples for pregnancy-associated glycoproteins detection test

Three experiments were performed to test if the time and storage conditions of milk samples, the preheating of samples in a water bath, as well as the carryover effect in laboratory analysis equipment could affect the pregnancy-associated glycoproteins (PAG) levels, and consequently the results of a...

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Bibliographic Details
Published in:Acta agriculturae Scandinavica. Section A, Animal science Animal science, 2017-04, Vol.67 (1-2), p.71-75
Main Authors: da Silva, H. K., Cassoli, L. D., Pantoja, J. C. F., Cerqueira, P. H. R., Winckler, J. P. P., Coitinho, T. B., Machado, P. F.
Format: Article
Language:English
Subjects:
Ambient temperature
Cow's milk
Enzyme-linked immunosorbent assay
Glycoproteins
Heating
Pregnancy
Storage
Storage conditions
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Summary:Three experiments were performed to test if the time and storage conditions of milk samples, the preheating of samples in a water bath, as well as the carryover effect in laboratory analysis equipment could affect the pregnancy-associated glycoproteins (PAG) levels, and consequently the results of a pregnancy test. The pregnancy test used in both experiments uses the enzyme-linked immunosorbent assay method to measure the concentrations of PAG and classify the samples as pregnant, nonpregnant or suspicious. As a result, PAG levels showed no variation when the samples were analyzed up to 9 days after collection, whether stored in ambient temperature or refrigerated. The preheating of the samples in a water bath and prior to the analysis of SCC and composition also did not affect the levels of PAG, allowing the same sample used in the quality analysis to be used for the pregnancy test.
ISSN:0906-4702
1651-1972
DOI:10.1080/09064702.2017.1395473