Anaerobik Rumen Fungus Caecomyces sp. GMLF12'nin Linoleate Isomerase Enzim Aktivitesinin Belirlenmesi

In this study, the presence of linoleate isomerase enzyme was investigated in Caecomyces sp. GMLF12. Linoleate isomerase enzyme enables the isomerisation of linoleic acid to conjugated linoleic acid. Caecomyces sp. GMLF12 isolate was inoculated to different concentrations of linoleic acid-containing...

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Published in:Kahramanmaraş Sütçü İmam Üniversitesi tarım ve doğa dergisi 2011-01, Vol.14 (4), p.12
Main Authors: ÇÖMLEKÇİOĞLU, Uğur, ÖZKÖSE, Emin, KAR, Bülent, AKYOL, İsmail, EKİNCİ, M
Format: Article
Language:Turkish
Subjects:
Acids
Cell surface
Enzymes
Fungi
Isomerization
Linoleate isomerase
Linoleic acid
Rhizoids
Spectrophotometry
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Summary:In this study, the presence of linoleate isomerase enzyme was investigated in Caecomyces sp. GMLF12. Linoleate isomerase enzyme enables the isomerisation of linoleic acid to conjugated linoleic acid. Caecomyces sp. GMLF12 isolate was inoculated to different concentrations of linoleic acid-containing media, but the fungi were not developed. Upon this, Caecomyces sp. GMLF12 was grown in glucose containing medium and spherical rhizoids were used as enzyme source. Cells were treated with linoleic acid, and enzymatic reaction was halted at given time intervals and CLA levels were measured by spectrophotometrically. The maximum CLA level was measured as 8.22 µg/ml at 45. min. The results showed that Caecomyces sp. GMLF12 have linoleate isomerase enzyme and this enzyme was found in cell surface.
ISSN:2619-9149
DOI:10.18016/ksujns.13858