Direct antifungal activity of tiadinil, a systemic acquired resistance inducer, and thymol formulations on Stagonosporopsis citrulli and control of watermelon gummy stem blight

• Published in: Journal of general plant pathology : JGPP 2018-07, Vol.84 (4), p.284-295
• Main Authors: Kefialew, Yonas, Kunwar, Sanju, Abate, Dawit, Ayalew, Amare, Colee, James, Ritchie, Laura, Olson, Stephen M., Paret, Mathews L.
• Format: Article
• Language: English
• Subjects: Agriculture; Antifungal activity; Biomedical and Life Sciences; Blight; Citrullus; Disease Control; Elongation; Essential oils; Foliar applications; Formulations; Fruits; Fungicides; Germination; Inoculation; Life Sciences; Microbiology; Mode of action; Mycelia; Pathogens; Plant diseases; Plant Pathology; Seedlings; Stem blight; Thymol
• ISSN: 1345-2630; 1610-739X
• DOI: 10.1007/s10327-018-0783-6

This study evaluated the direct antifungal activity of tiadinil [ N -(3-chloro-4-methylphenyl)-4-methyl-1,2,3-thiadiazole-5-carboxamide], a systemic acquired resistance (SAR) inducer and two formulations of thymol (thymol I and thymol II) against Stagonosporopsis citrulli , the causal agent of gummy stem blight (GSB) disease of watermelon. Tiadinil, thymol I and thymol II completely inhibited the mycelial growth of S. citrulli in vitro at ≥ 100 ppm. Conidial germination and germ tube elongation were completely inhibited by tiadinil at ≥ 2000 ppm and by thymol-based formulations at ≥ 100 ppm. A single foliar application of tiadinil at ≥ 10 ppm or a single application of thymol I and II at ≥ 1 ppm, 48 h before or after pathogen inoculation, significantly reduced disease severity of watermelon seedlings inoculated with 10 5 /ml conidial suspension of S. citrulli , compared to respective nontreated controls. Plants treated with foliar application of tiadinil at ≥ 1000 ppm before pathogen inoculation had significantly lower disease severity than plants that received an equivalent drench application. The efficacy of foliar application of tiadinil was affected by concentration and frequency of application. The study suggests direct antifungal activity of tiadinil, indicating a new mode of action of tiadinil against GSB disease of watermelon. The study also demonstrated direct antifungal action of thymol, a formulated active compound of essential oils, against S. citrulli and GSB disease of watermelon.