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Bioreactor Analysis for the Corn-Cob Valorization in the Xylanase Production
Purpose To achieve the optimization of the solid-state fermentation (SSF) processes where agro-industrial wastes are used, a better understanding of biological aspects (mathematical modeling) are needed, due the heterogeneity of these materials. The purpose of this study was to compare the effect of...
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Published in: | Waste and biomass valorization 2018-06, Vol.9 (6), p.995-1001 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Purpose
To achieve the optimization of the solid-state fermentation (SSF) processes where agro-industrial wastes are used, a better understanding of biological aspects (mathematical modeling) are needed, due the heterogeneity of these materials. The purpose of this study was to compare the effect of the bioreactor arrangement (Tray and Column) in the synthesis of xylanase by
Rhizomucor pusillus
strain in SSF process.
Methods
Fermentation process was realized in both, tray and column bioreactors, using corn-cob (CC) as a carrier-substrate. Tray bioreactor and packed-bed bioreactor, containing fungal spores, 3 g of CC as substrate and support, and 8 mL of minimum Czapek-Dox culture medium; were incubated at 50 °C for 5 days. Maximal specific growth rate (
μ
max
), maximal biomass level (
X
max
), product/biomass yield (
α
) and secondary coefficient of product formation or destruction (
β
) were estimated by mathematical models.
Results
High value of xylanase enzyme activity (587.6 U/gds) was found in the column bioreactor. The logistic equation for cell growth and Luedeking-Piret equation for xylanase production could satisfactorily simulate the bioreactors’ kinetics data.
Conclusions
The production of xylanase by
Rhizomucor pusillus
SOC-4A is affected by the type of bioreactor. Data showed that the most efficient bioreactor in production of the xylanase enzyme is the column bioreactor. |
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ISSN: | 1877-2641 1877-265X |
DOI: | 10.1007/s12649-016-9754-3 |