Evaluation of process conditions in the performance of yeast on alcoholic fermentation

This work studied the resistance of Saccharomyces cerevisiae Y904 to ethanol on an alcoholic fermentation process operated in fed-batch. The effect of temperature, inoculum size and substrate concentration on fermentation yield, productivity and residual sugars concentration was studied by a central...

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Bibliographic Details
Published in:Chemical engineering communications 2018-06, Vol.205 (6), p.846-855
Main Authors: Cruz, Mariana Lopes, Resende, Miriam Maria de, Ribeiro, Eloízio Júlio
Format: Article
Language:English
Subjects:
Baking yeast
Ethanol
Fermentation
Inoculum
Productivity
Substrates
Sugar
Sugarcane
Temperature effects
Yeast
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Summary:This work studied the resistance of Saccharomyces cerevisiae Y904 to ethanol on an alcoholic fermentation process operated in fed-batch. The effect of temperature, inoculum size and substrate concentration on fermentation yield, productivity and residual sugars concentration was studied by a central composite design (CCD). Based on the CCD study, it was determined the optimum values of 240, 35 g/L, and 26°C for total reducing sugars, inoculum concentration and temperature, respectively. This set of conditions experimentally enabled a productivity of 6.0 g/L h, a yield of 93% and an alcohol content of 113.6 g/L, after 10 h of fermentation. When yeast cells were adapted at 4°C, the inoculum pH adjusted to 2.5 and sugarcane broth used as substrate, a 94% yield and a 10.1 g/L. h productivity were achieved.
ISSN:0098-6445
1563-5201
DOI:10.1080/00986445.2017.1423061