Characterization of Capsicum annuum L. leaf and root antimicrobial peptides: antimicrobial activity against phytopathogenic microorganisms

• Published in: Acta physiologiae plantarum 2018-06, Vol.40 (6), p.1-15, Article 107
• Main Authors: da Silva Pereira, Lídia, do Nascimento, Viviane Veiga, de Fátima Ferreira Ribeiro, Suzanna, Rodrigues, Rosana, Fernandes, Katia Valevski Sales, de Oliveira Carvalho, André, Vasconcelos, Ilka Maria, dos Santos Bento, Cíntia, Sudré, Cláudia Pombo, Zottich, Umberto, Gomes, Valdirene Moreira
• Format: Article
• Language: English
• Subjects: Acids; Agriculture; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Antimicrobial peptides; Biomedical and Life Sciences; Capsicum annuum; Colletotrichum gloeosporioides; Colletotrichum lindemuthianum; Electrophoresis; Fungi; Gels; High performance liquid chromatography; Leaves; Life Sciences; Liquid chromatography; Microorganisms; Original Article; Pathogens; Peppers; Peptides; Pest control; Pests; Phytopathogenic fungi; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant tissues; Proteins; Roots; Trypsin; Xanthomonas euvesicatoria; α-Amylase
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-018-2685-9

This study aimed to detect and characterize antimicrobial proteins, especially antimicrobial peptides (AMPs) from leaves and roots of Capsicum annuum and to evaluate their inhibitory activities against different phytopathogenic fungi and the bacterium Xanthomonas euvesicatoria . Two methodologies were used for the extraction of peptides from leaves and roots of C. annuum : acid and ethanolic extraction. Extracts were subjected to reversed-phase chromatography on HPLC. The extraction and purification procedures were analysed by uni- and bi-dimensional electrophoresis in tricine gels. Our results show that alcoholic and acid extracts from both tissues can inhibit the growth of the phytopathogenics fungi C. lindemuthianum and C. gloeosporioides . The acid extracts from both tissues are active against X. euvesicatoria and only leaf extracts displayed specific inhibitory activity towards trypsin and α-amylase activity. The data compiled here aim to contribute to establish the multiplicity of potential uses of plant AMPs for the control of pests and pathogens of agricultural relevance.