Association between g.98535683A > G:BTAU7 marker the CAST gene and meat characteristics of Nellore cattle (Bos indicus) and their crosses with Bos taurus

In meat science, single nucleotide polymorphisms (SNPs) have contributed to the rapid identification of genetic markers in the selection and improvement of quality characteristics. The calpastatin protein (CAST) inhibits the activity of calpain and it regulates the tenderization of the meat during t...

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Bibliographic Details
Published in:Journal of animal science 2016-09, Vol.94, p.141-142
Main Authors: Chardulo, L A L, Curi, R A, Oliveira, H N, Silva, J A I V, Pereira, G L, Enriquez-Valencia, C E, Malheiros, J M, Nadalini, E C
Format: Article
Language:English
Subjects:
Alleles
Beef
Beef cattle
Bos taurus
Bovidae
Calpain
Calpastatin
Cattle
Gene frequency
Gene polymorphism
Genetic markers
Genotypes
Lipids
Meat
Meat quality
Molecular chains
Polymorphism
Populations
Proteins
Single-nucleotide polymorphism
Tenderization
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Summary:In meat science, single nucleotide polymorphisms (SNPs) have contributed to the rapid identification of genetic markers in the selection and improvement of quality characteristics. The calpastatin protein (CAST) inhibits the activity of calpain and it regulates the tenderization of the meat during the ageing process. The SNP g.98535683A > G:BTAU7 the CAST gene was associated with meat tenderness in the Bos taurus breeds. However, before transferring the populations' molecular marker where they were identified to other populations, corroboration of their effects on characteristics of interest is required in different breeds and environments. The aim of this study was to estimate allelic and genotypic frequencies of the SNP g.98535683A > G:BTAU7 the CAST gene and evaluate the occurrence of associations between this polymorphism and meat characteristics in Bos indicus. Were used five hundred Nellore cattle (n = 300) and crosses (n = 200) with Bos taurus from six different genetic groups and genotyped for the SNP g.98535683A > G: BTAU7 and phenotyped to shear force (SF), myofibrillar fragmentation index (MFI) rib eye area (REA), backfat thickness (BFT) and total lipids (LIP). Both alleles from this SNP were found, the A allele and the G allele with their genotypes AA, AG and GG. In the six genetic groups studied the A allele showed higher frequency than the allele G. There were no differences in allele frequencies between the genetic groups studied, showing that should be similar in Bos indicus and Bos taurus. Significant and close association between the SNP g.98535683A > G:BTAU7 and tenderness (MFI, p = 0.0044; SF, p = 0.0598) was observed where the AA genotype was better for this characteristics. The SNP g.98535683A > G:BTAU7 showed no significant association with the characteristics of REA, BFT and LIP. These results showed the occurrence of the SNP g.98535683A > G:BTAU7 the CAST gene in Nellore (Bos indicus) and its potential application in animal selection for the tenderness in this breed and its crosses. Financial support: FAPESP.
ISSN:0021-8812
1525-3163