Pre-treatment with melatonin decreases abamectin induced toxicity in a nocturnal insect Spodoptera litura (Lepidoptera: Noctuidae)

• Published in: Environmental toxicology and pharmacology 2017-12, Vol.56, p.76-85
• Main Authors: Subala, Subramanian P., Zubero, Eduardo E., Alatorre-Jimenez, Moises A., Shivakumar, Muthugounder S.
• Format: Article
• Language: English
• Subjects: Abamectin; Animals; Antioxidant enzyme; Antioxidants; Artificial diets; Carbonyls; Diet; Exposure; Free radicals; Gene Expression Regulation - drug effects; Hemolymph; Impact analysis; Insect Proteins - metabolism; Insecticides; Insecticides - toxicity; Insects; Intestines - drug effects; Intestines - metabolism; Ivermectin - analogs & derivatives; Ivermectin - toxicity; Larvae; Lipid peroxidation; Lipid Peroxidation - drug effects; Melatonin; Melatonin - pharmacology; Midgut; Nocturnal; Oxidative stress; Oxidative Stress - drug effects; Pesticide toxicity; Pesticides; Pretreatment; Reactive oxygen species; Reactive Oxygen Species - metabolism; Spodoptera - drug effects; Spodoptera - growth & development; Studies; Time dependence; Time Factors; Toxicity
• ISSN: 1382-6689; 1872-7077
• DOI: 10.1016/j.etap.2017.08.025

[Display omitted] •Oxidative stress is an important component of the mechanism of pesticide toxicity.•To investigate time-dependent melatonin effects against abamectin oxidative stress in S.litura.•ABA group showed significant changes in the ROS and carbonyl content in midgut.•PM showed increase of SOD, GST, CAT, POX, APOX levels in midgut.•Indoleamine can play a vital role curtailing the abamectin toxicity in time dependent. Oxidative stress is an important component of the mechanism of pesticide toxicity. The aim of the present study was to investigate the time-dependent melatonin effects against abamectin-induced oxidative stress in a S.litura model. Larvae were divided into 5 different groups; (1) control group,(2) Melatonin group (4.3×10−5M/100ml diet), (3) Abamectin group 1.5ml/L, (4) Pre-melatonin treated group (PM) (4.3×10−5M/100ml diet) before abamectin exposure 1.5ml/L, (5) Post-melatonin treated group (TM) after abamectin exposure. Melatonin was supplemented via artificial diet in PM and TM animals during 24h. Midgut, fatbody, and hemolymph, were collected for the analysis of oxidative stress markers (Total ROS, GSH, nitrite, TBARS, LPO), antioxidant enzyme levels (SOD, GST, CAT, POX, APOX) in fifth instar larvae. Midgut damage was examined by using morphological analysis. Our results observed that ABA group showed significant changes (p