The First Recombinant Viper Three-Finger Toxins: Inhibition of Muscle and Neuronal Nicotinic Acetylcholine Receptors

Genes encoding two three-finger toxins TFT-AF and TFT-VN, nucleotide sequences of which were earlier determined by cloning cDNA from venom glands of vipers Azemiops feae and Vipera nikolskii , respectively, were expressed for the first time in E. coli cells. The biological activity of these toxins w...

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Published in:Doklady. Biochemistry and biophysics 2018-03, Vol.479 (1), p.127-130
Main Authors: Makarova, Ya. V., Kryukova, E. V., Shelukhina, I. V., Lebedev, D. S., Andreeva, T. V., Ryazantsev, D. Yu, Balandin, S. V., Ovchinnikova, T. V., Tsetlin, V. I., Utkin, Yu. N.
Format: Article
Language:English
Subjects:
Acetylcholine receptors (nicotinic)
Biochemistry
Biological activity
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Calcium
Calcium imaging
Chemical bonds
Cloning
E coli
Electric instruments
Enzymes
Life Sciences
Molecular Biology
Nervous system
Neuroblastoma
Neurotoxicity
Nicotine
Proteins
Snakes
Toxins
Venom gland
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Summary:Genes encoding two three-finger toxins TFT-AF and TFT-VN, nucleotide sequences of which were earlier determined by cloning cDNA from venom glands of vipers Azemiops feae and Vipera nikolskii , respectively, were expressed for the first time in E. coli cells. The biological activity of these toxins was studied by electrophysiological techniques, calcium imaging, and radioligand analysis. It was shown for the first time that viper three-finger toxins are antagonists of nicotinic acetylcholine receptors of neuronal and muscle type.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672918020205