The antioxidant and cytoprotective activity of Ocimum gratissimum extracts against hydrogen peroxide-induced toxicity in human HepG2 cells

Ocimum gratissimum is used as a traditional folk medicine in many countries. The objective of this study was to evaluate the antioxidant activities of an aqueous O. gratissimum extract (OGE) and to evaluate its cytoprotective activity against hydrogen peroxide-induced toxicity in human HepG2 cells....

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Published in:Yàowu shi͡p︡in fenxi 2013-09, Vol.21 (3), p.253-260
Main Authors: Chiu, Yung-Wei, Lo, Hung-Jen, Huang, Hsin-Yu, Chao, Pei-Yu, Hwang, Jin-Ming, Huang, Pei-Yun, Huang, Shyh-Jer, Liu, Jer-Yuh, Lai, Te-Jen
Format: Article
Language:English
Subjects:
Antioxidants
Calibration
Cancer
Chemical compounds
Cytotoxicity
Free radical scavenging activity
Free radicals
Herbal medicine
Hydrogen peroxide
Lipid peroxidation
Lipids
Liver
Liver diseases
Medical research
Ocimum
Ocimum gratissimum
Oxidative stress
Pharmacology
Phenolic compounds
Phenols
Phytochemicals
Potassium
Pretreatment
Thiobarbituric acid
Toxicity
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Summary:Ocimum gratissimum is used as a traditional folk medicine in many countries. The objective of this study was to evaluate the antioxidant activities of an aqueous O. gratissimum extract (OGE) and to evaluate its cytoprotective activity against hydrogen peroxide-induced toxicity in human HepG2 cells. The results revealed that the total phenolic content of the OGE reached 20%. In the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the OGE reduced up to 80% of the free radicals at a plateau concentration of 66.7 μg/mL. This indicates that OGE contains considerable free radical scavenging activity. In a dose-dependent manner, OGE pretreatment counteracted the decrease in cell viability in H2O2-treated HepG2 cells (p 
ISSN:1021-9498
2224-6614
DOI:10.1016/j.jfda.2013.07.002