Maximization of hydrogen fermentative process from delignified water hyacinth using sodium chlorite

• Published in: Energy conversion and management 2018-02, Vol.157, p.257-265
• Main Authors: Elsamadony, Mohamed, Tawfik, Ahmed
• Format: Article
• Language: English
• Subjects: Acetic acid; Aquatic plants; Bacteria; Biodegradation; Cellulase; Cellulose; Clostridium; Delignification; Destruction; Dosage; Enterobacter; Enzymes; Fermentation; Floating plants; Hemicellulose; Hydrogen; Hydrogen production; Lignin; Phenolic compounds; Phenols; Polysaccharides; Pretreatment; Pretreatment of water; Sodium; Sodium chlorite; Water hyacinth; Water hyacinths; Xylanase
• ISSN: 0196-8904; 1879-2227
• DOI: 10.1016/j.enconman.2017.12.013

[Display omitted] •8 mg/L of sodium chlorite enhanced H2 yield to 119.6 ± 7.8 mL/gTVS.•Sodium chlorite exhibited higher lignin destruction.•Phenolic compounds inhibit enzyme activities and bacterial counts.•Maximum profit was 0.80 $/gWH/day using 8.0 mg NaClO2/L. This study investigated the direct supplementation of sodium chlorite (NaClO2) in acidic fermentation process rather than using NaClO2 as a pretreatment agent. This approach targets saving both of the cost of external acetic acid that needs to be added in pretreatment and the loss of polysaccharide, which simultaneously occurs during pretreatment process. Whereas, fermentation of water hyacinth (WH) provided quite low H2 yield (HY) of 39.7 ± 2.5 mL/gTVS and lignin destruction of 2.6 ± 0.2%. Cellulase and xylanase enzymes amounted to 2.15 ± 0.18 and 1.81 ± 0.14 U/mL, respectively. Enterobacter and Clostridium sp. accounted for 2.41 ± 0.15 × 105 and 4.02 ± 0.27 × 104 cfu/mL, respectively. However, NaClO2 addition significantly augmented HY, cellulase and xylanase enzymes to 119.6 ± 7.8 mL/gTVS, 3.46 ± 0.21 and 2.09 ± 0.23 U/mL at dosage of 8.0 mg/L, respectively. Moreover, cellulose, hemicellulose and lignin degradation efficiencies were maximized to 57.8 ± 3.1, 46.3 ± 3.8 and 31.6 ± 1.6%, respectively. Enterobacter and Clostridium sp. counts were 4.23 ± 0.28 × 105 and 9.75 ± 0.52 × 104 cfu/mL, respectively in the batches supplemented with NaClO2. Nevertheless, at a dosage exceeding 8.0 mg/L, the HY (26.1 ± 2.0 mL/gTVS) and bacterial count was highly deteriorated due to the excessive production of inhibitory phenolic compounds of 1.61 ± 0.12 g/L in the medium where the destruction of lignin was quite high (38.8 ± 2.4%).