Glioblastoma Multiforme stem cells are highly sensitive to some human non-pathogenic enteroviruses

• Published in: Journal of pharmaceutical sciences and research 2018-04, Vol.10 (4), p.936-939
• Main Authors: Soboleva, A V, Seryak, D A, Gabdrakhmanova, A F, Sosnovtseva, A O, Tkhe, L H, Kochetkov, D V, Ilyinskaya, G V, Golbin, D A, Lipatova, A V, Chumakov, P M
• Format: Article
• Language: English
• Subjects: Brain cancer; Cell culture; Chemotherapy; Disease; Immunotherapy; Molecular biology; Penicillin; Stem cells; Tumors; Vaccines; Viruses
• ISSN: 0975-1459

For these reasons, after any course of therapy, recurrence of the disease is almost inevitable [7]. [...]it is especially important to search for therapeutic effects that can effectively destroy glioblastoma stem cells without showing significant toxicity to normal cells and tissues [8-10]. The cells were then suspended in 15 ml of Neurosphere medium that constituted Neurobasal Medium (Life Technologies) supplemented with 1· B27 without vitamin A (Thermo Fisher), 2 mM glutamine, 1 mM sodium pyruvate, 10 ng/ml basic fibroblast growth factor (bFGF), and 10 ng/ml of epidermal growth factor (EGF, PanEco, Moscow) and 100 pg / ml of penicillin and streptomycin, the number of cells was counted, adjusted to 4 x 105 cells / ml and dispersed in 1.5 ml per well in 6-well plates. The following enterovirus strains were used in the study: vaccine Sabin strain of poliovirus type 1 (PVS1), and four human enterovirus strains isolated from healthy individuals [16, 20] - Coxsackievirus A7 (LEV8 strain), Coxsackievirus B5 (LEV14 strain), Echovirus 12 (LEV7 strain) were propagated in RD cells and stores as clarified cell culture supernatants at - 20°C. Virus titers were determined by infecting monolayers of RD cells with serial dilutions of the virus stock in 96-well plates. Because of the variable growth rate of neurospheres we were able to select 5 neurosphere cultures suitable for further analysis.