HPLC study for evaluating the significance of pH in the inhibiting effect of phosphate buffer on the leaching pattern of resin composites

• Published in: Journal of liquid chromatography & related technologies 2018-05, Vol.41 (8), p.430-436
• Main Authors: Mourouzis, Petros, Samanidou, Victoria, Koulaouzidou, Elisabeth A., Palaghias, Georgios
• Format: Article
• Language: English
• Subjects: Buffer solutions; Composite materials; Distilled water; High performance liquid chromatography; Leaching; Liquid chromatography; pH effects; Phosphate; Phosphates; Polymer matrix composites; Saliva; Silk; Storage
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826076.2018.1441157

An HPLC method was applied to evaluate the inhibiting effect of phosphate buffer on the leaching pattern of resin composites and the significance of pH levels during the storage period in the solutions. Ten (10) disk-shaped specimens (2 mm × 12 mm diameter) from Evetric restorative resin composite were immersed by means of a silk string in each of the following solutions: Distilled water (solution A), phosphate buffer of pH = 4.5 (solution B) and phosphate buffer of pH = 6.8 (solution C). After storage for 7, 30 and 60 days' period, the eluates were examined by means of High Performance Liquid Chromatography (HPLC) at each time interval. The storage of the resin composite specimens in the phosphate buffer solution demonstrated an inhibiting effect on the leaching pattern compared to the distilled water solution. The results of this study suggest that HPLC is a useful tool to investigate the effect of the pH value of phosphate buffer solution, which resembles saliva, on the leaching pattern of resin composites.